Design of a cellulose-based nanocomposite as a potential polymeric scaffold in tissue engineering

Pooyan, Parisa; Kim, Il Tae; Jacob, Karl I.; Tannenbaum, Rina; Garmestani, Hamid

doi:10.1016/j.polymer.2013.01.030

Cited by 36 publications

(21 citation statements)

References 52 publications

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“…16,17,22 Its unique properties have sustained the elevator pitch of several BC applications, especially in the biomedical field, where temporary skin substitutes and artificial blood vessels appear as patented products (such as Biofill and BASYC). Recent studies on the potential use of BC as a biomaterial include artificial skin 17 , vascular grafts 20,23,24 , conduits in urinary reconstruction and diversion 25 , cartilage replacement 26 , bone regeneration 27 , artificial cornea 28 , tissue engineering hydrogels 29 and scaffolds 30 . Also BC is not biodegradable in the human body, which can be beneficial since substrates developed with degradable materials and biological tissues can be difficult to handle and may induce retinal degeneration due to material degradation.…”

Section: Introductionmentioning

confidence: 99%

“…Also BC is not biodegradable in the human body, which can be beneficial since substrates developed with degradable materials and biological tissues can be difficult to handle and may induce retinal degeneration due to material degradation. [30][31][32][33][34][35][36] In this work, we evaluated the ability of RPE cells to adhere and grow on BC-based substrates.…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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“…1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Generally, the interfacial adhesion between CNCs and a polymer matrix is one of the most important factors affecting nanocomposite properties. In order to enhance the dispersion of CNCs and increase the interfacial strength between the two phases, various methods have been attempted, including magnetic field alignment within the matrix 106 , use of plasticizers 107 and surfactants 108,109 , or through surface modifications 110,111 . A quite extensive range of polymer matrices, both synthetic and natural based, has been used to produce nanocomposite films and membranes containing CNCs, which will be described in the following sections.…”

Section: Dense Films and Membranesmentioning

confidence: 99%

“…Thin films were produced by solvent casting and, according to the authors, the obtained bionanocomposites exhibited excellent mechanical performance at body temperature, claiming that CNCs impart significant strength and directional rigidity even at 0.2 wt.% (about twofold higher compared to CAP) and almost double that at only 3.0 wt.%. In a following study, these authors explored the same concept but aligned the CNCs embedded within the CAP matrix by applying a relatively weak external magnetic field (0.3 T) 106 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 microstructural point of view, the composites exhibited a relatively controlled porous structure (average pore diameter of 3 µm and a void fraction of ∼13%, for a 0.2 wt.% CNCs loading), which could be potentially helpful for cell seeding and proliferation because it would allow diffusion of fluids and gases deep into the material 106 . Furthermore, the alignment of the CNCs also confers an oriented microporous nanostructure to the system that could potentially induce contact guidance to cells in the direction of the aligned CNCs 106 .…”

Section: Natural-based Polymeric Systemsmentioning

confidence: 99%

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

2014

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Cellulose nanocrystals (CNCs) are a renewable nanosized raw material that is drawing a tremendous level of attention from the materials community. These rod-shaped nanocrystals that can be produced from a variety of highly available and renewable cellulose-rich sources are endowed with exceptional physicochemical properties which have promoted their intensive exploration as building blocks for the design of a broad range of new materials in the past few decades. However, only recently have these nanosized substrates been considered for bioapplications following the knowledge on their low toxicity and ecotoxicological risk. This Review provides an overview on the recent developments on CNC-based functional biomaterials with potential for tissue engineering (TE) applications, focusing on nanocomposites obtained through different processing technologies usually employed in the fabrication of TE scaffolds into various formats, namely, dense films and membranes, hierarchical three-dimensional (3D) porous constructs (micro/nanofibers mats, foams and sponges), and hydrogels. Finally, while highlighting the major achievements and potential of the reviewed work on cellulose nanocrystals, alternative applications for some of the developed materials are provided, and topics for future research to extend the use of CNCs-based materials in the scope of the TE field are identified.

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“…[ 19 ] The shear‐thinning and self‐healing behavior associated with this hydrogel [ 18 ] is due to the entanglement, hydrogen bonds, and ionic interactions that keep it together, [ 17 ] which also prevent degradation in aqueous environments. [ 20 ] The CNF is naturally biocompatible [ 12,21 ] and has therefore been used for different tissue engineering applications. Among those with promising results are cultures in CNF hydrogel of stem cells, [ 12 ] culture, [ 6 ] and differentiation [ 8 ] of liver cell lines such as HepaRG and HepG2 or progenitor cell thereof and as a sacrificial template for the engineering of tubular cell constructs.…”

Section: Introductionmentioning

confidence: 99%

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

Krüger

Oosterhoff

Wolferen

et al. 2020

Adv Healthcare Materials

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To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting. Recently, adult liver‐derived liver organoids are found to be of much interest due to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte‐like fate. The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable. Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids. The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte‐like cells compared to MG. Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.

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Design of a cellulose-based nanocomposite as a potential polymeric scaffold in tissue engineering

Cited by 36 publications

References 52 publications

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

The Potential of Cellulose Nanocrystals in Tissue Engineering Strategies

Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids

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