Electron beam collimators for nonâstandard field sizes and shapes are typically fabricated using Styrofoam molds to cast the aperture cutâout. These molds are often produced using a dedicated foam cutter, which may be expensive and only serves a single purpose. An increasing number of radiotherapy departments, however, has a 3D printer onâsite, to create a wide range of customâmade treatment auxiliaries, such as bolus and dosimetry phantoms. The 3D printer can also be used to produce patientâspecific aperture cutâouts, as elaborated in this note. Openâsource programming language was used to automatically generate the mold's shape in a generic digital file format readable by 3D printer software. The geometric mold model has the patient's identification number integrated and is to be mounted on a uniquely fitting, reusable positioning device, which can be 3D printed as well. This assembly likewise fits uniquely onto the applicator tray, ensuring correct and errorâfree alignment of the mold during casting of the aperture. For dosimetric verification, two aperture cutâouts were cast, one using a conventionally cut Styrofoam mold and one using a 3D printed mold. Using these cutâouts, the clinical plan was delivered onto a phantom, for which the transversal dose distributions were measured at 2Â cm depth using radiochromic film and compared using gammaâindex analysis. An agreement score of 99.9% between the measured 2D dose distributions was found in the (10%â80%) dose region, using 1% (local) doseâdifference and 1.0Â mm distanceâtoâagreement acceptance criteria. The workflow using 3D printing has been clinically implemented and is in routine use at the author's institute for all patientâspecific electron beam aperture cutâouts. It allows for a standardized, costâeffective, and operatorâfriendly workflow without the need for dedicated equipment. In addition, it offers possibilities to increase safety and quality of the process including patient identification and methods for accurate mold alignment.