Design of a Homogeneous Multifunctional Supported Lipid Membrane on Layer-by-Layer Coated Microcarriers

Göse, Martin; Pescador, Paula; Reibetanz, Uta

doi:10.1021/bm5016688

Cited by 10 publications

(5 citation statements)

References 69 publications

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“…However, the main advantage of LbL carriers is their modular design, which allows for an independent surface modification. 45 This could also include the presence of viral proteins in their outermost layer, such that advantages of both systems can be combined. Additionally, as iPSC colonies respond differently to LbL carriers and CoxB3 virus particles over time of cultivation, they might be applied in a complementary approach at different time points.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to artificial drug delivery systems, viral particles possess a natural surface functionalization to facilitate efficient cellular uptake. However, the main advantage of LbL carriers is their modular design, which allows for an independent surface modification . This could also include the presence of viral proteins in their outermost layer, such that advantages of both systems can be combined.…”

Section: Discussionmentioning

confidence: 99%

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Influence of Growth Characteristics of Induced Pluripotent Stem Cells on Their Uptake Efficiency for Layer-by-Layer Microcarriers

et al. 2016

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Induced pluripotent stem cells (iPSCs) have the ability to differentiate into any specialized somatic cell type, which makes them an attractive tool for a wide variety of scientific approaches, including regenerative medicine. However, their pluripotent state and their growth in compact colonies render them difficult to access and, therefore, restrict delivery of specific agents for cell manipulation. Thus, our investigation focus was set on the evaluation of the capability of layer-by-layer (LbL) designed microcarriers to serve as a potential drug delivery system to iPSCs, as they offer several appealing advantages. Most notably, these carriers allow for the transport of active agents in a protected environment and for a rather specific delivery through surface modifications. As we could show, charge and mode of LbL carrier application as well as the size of the iPSC colonies determine the interaction with and the uptake rate by iPSCs. None of the examined conditions had an influence on iPSC colony properties such as colony morphology and size or maintenance of pluripotent properties. An overall interaction rate of LbL carriers with iPSCs of up to 20% was achieved. Those data emphasize the applicability of LbL carriers for stem cell research. Additionally, the potential use of LbL carriers as a promising delivery tool for iPSCs was contrasted to viral particles and liposomes. The identified differences among those delivery tools have substantiated our major conclusion that LbL carrier uptake rate is influenced by characteristic features of the iPSC colonies (most notably colony size) in addition to their surface charges.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of Growth Characteristics of Induced Pluripotent Stem Cells on Their Uptake Efficiency for Layer-by-Layer Microcarriers

et al. 2016

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“…The relevance of the last coating layer in governing the interactions with the tissue/cell of interest with LbL systems has been under broad investigation . The following section is dedicated to the use of specific coating materials for the last coating layer in relation to LbL‐coated solid drug cores.…”

Section: Coating Materials and Lbl Methodologymentioning

confidence: 99%

Layer‐by‐Layer Coating of Solid Drug Cores: A Versatile Method to Improve Stability, Control Release and Tune Surface Properties

Połomska

Leroux

2016

Macromolecular Bioscience

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Layer-by-layer coating is a simple and versatile technique based on the sequential deposition of molecular species on planar surfaces or colloidal templates. Its relevance in drug delivery primarily emerges from its versatility to control the release rate of the cargo encapsulated within the colloidal core. The focus of this review is the layer-by-layer encapsulation of colloidal particles purely composed of drug, including the core fabrication step, coating materials and techniques, multilayer shell permeability control, and reported in vitro and in vivo outcomes.

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“…Lipid concentration in the extruded samples was determined by Bartlett assay (40) with slight modifications (41). Briefly, liposomes were incinerated in HClO 4 (70%, v/v; 90 min at 230°C), ascorbic acid (700 l; 3%, v/v) and ammonium molybdate solution (700 l; 3.6 mM in 12.5% perchloric acid, v/v) were added, and samples were incubated for 5 min at 100°C.…”

Section: Lipid Quantificationmentioning

confidence: 99%

Cytochrome c autocatalyzed carbonylation in the presence of hydrogen peroxide and cardiolipins

Barayeu

Lange

Méndez

et al. 2019

Journal of Biological Chemistry

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Edited by Ruma BanerjeeCytochrome c (cyt c) is a small hemoprotein involved in electron shuttling in the mitochondrial respiratory chain and is now also recognized as an important mediator of apoptotic cell death. Its role in inducing programmed cell death is closely associated with the formation of a complex with the mitochondrionspecific phospholipid cardiolipin (CL), leading to a gain of peroxidase activity. However, the molecular mechanisms behind this gain and eventual cyt c autoinactivation via its release from mitochondrial membranes remain largely unknown. Here, we examined the kinetics of the H 2 O 2 -mediated peroxidase activity of cyt c both in the presence and absence of tetraoleoyl cardiolipin (TOCL)-and tetralinoleoyl cardiolipin (TLCL)-containing liposomes to evaluate the role of cyt c-CL complex formation in the induction and stimulation of cyt c peroxidase activity. Moreover, we examined peroxide-mediated cyt c heme degradation to gain insights into the mechanisms by which cyt c self-limits its peroxidase activity. Bottom-up proteomics revealed >50 oxidative modifications on cyt c upon peroxide reduction. Of note, one of these by-products was the Tyr-based "cofactor" trihydroxyphenylalanine quinone (TPQ) capable of inducing deamination of Lys ⑀-amino groups and formation of the carbonylated product aminoadipic semialdehyde. In view of these results, we propose that autoinduced carbonylation, and thus removal of a positive charge in Lys, abrogates binding of cyt c to negatively charged CL. The proposed mechanism may be responsible for release of cyt c from mitochondrial membranes and ensuing inactivation of its peroxidase activity.Cytochrome c (cyt c) 4 was originally identified as an electron shuttle within the respiratory chain, essentially contributing to the aerobic pathway of oxidative phosphorylation. However, the single-chain hemoprotein is nowadays also known as a key mediator of apoptosis (1), making cyt c an important cornerstone of the cell fate. Upon apoptosis induction, the protein is released from the inner membrane space of the mitochondria to the cytosol where it binds to apoptosis-activating factor 1 (2), leading to caspase-9 activation (3) and subsequently apoptosis. Thereby, cyt c seems to actively contribute to this process, although the exact biochemical mechanisms for its release from the mitochondria are still under discussion.Although cyt c-mediated pore formation leading to outer mitochondrial membrane permeabilization was suggested (4), others did not observe a considerable unfolding and/or insertion of the protein into the outer mitochondrial membrane (5,6). Another mechanism proposed by Kagan et al. (7) links the release of cyt c at the early stage of apoptosis to the gain of a peroxidase activity by this protein. Cyt c binds to cardiolipin (CL), a mitochondrion-specific phospholipid, which redistributes from the inner to the outer mitochondrial membrane upon apoptosis onset (8 -10). This complex formation results in structural modifications of the protein that trigger...

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Design of a Homogeneous Multifunctional Supported Lipid Membrane on Layer-by-Layer Coated Microcarriers

Cited by 10 publications

References 69 publications

Influence of Growth Characteristics of Induced Pluripotent Stem Cells on Their Uptake Efficiency for Layer-by-Layer Microcarriers

Influence of Growth Characteristics of Induced Pluripotent Stem Cells on Their Uptake Efficiency for Layer-by-Layer Microcarriers

Layer‐by‐Layer Coating of Solid Drug Cores: A Versatile Method to Improve Stability, Control Release and Tune Surface Properties

Cytochrome c autocatalyzed carbonylation in the presence of hydrogen peroxide and cardiolipins

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