Design of a Human Rhinovirus-14 3C Protease-Inducible Caspase-3

Wagner, Hanna J.; Weber, Wilfried

doi:10.3390/molecules24101945

Cited by 6 publications

(6 citation statements)

References 31 publications

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“…10 ) [ 165 ]. In this study, four different switch mechanisms were used: (i) protease-sensitive linkers for crosslinking polyacrylamide to a hydrogel, (ii) protease-sensitive linkers to couple a protease or an output protein to a polymer network, (iii) inactive proteases that were activatable by cleavage with another protease [ 187 ], and (iv) small molecule-responsive affinity-based linkers to immobilize and competitively release proteins to/from a polymer network (for example, the immobilization of GyrB-tagged proteins to novobiocin-functionalized polymer). Three proteases with orthogonal target specificity were used: the TEV protease, the human rhinovirus 3C protease, and caspase-3.…”

Section: Engineering Cells To Synthesize (Precursors Of) Non-living Materialsmentioning

confidence: 99%

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Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

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Materials in nature have fascinating properties that serve as a continuous source of inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches have yielded remarkable structural and functional materials for a plethora of applications. Despite these advances, many properties of natural materials remain challenging or yet impossible to incorporate into synthetic materials. Natural materials are produced by living cells, which sense and process environmental cues and conditions by means of signaling and genetic programs, thereby controlling the biosynthesis, remodeling, functionalization, or degradation of the natural material. In this context, synthetic biology offers unique opportunities in materials sciences by providing direct access to the rational engineering of how a cell senses and processes environmental information and translates them into the properties and functions of materials. Here, we identify and review two main directions by which synthetic biology can be harnessed to provide new impulses for the biologization of the materials sciences: first, the engineering of cells to produce precursors for the subsequent synthesis of materials. This includes materials that are otherwise produced from petrochemical resources, but also materials where the bio-produced substances contribute unique properties and functions not existing in traditional materials. Second, engineered living materials that are formed or assembled by cells or in which cells contribute specific functions while remaining an integral part of the living composite material. We finally provide a perspective of future scientific directions of this promising area of research and discuss science policy that would be required to support research and development in this field.

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Section: Engineering Cells To Synthesize (Precursors Of) Non-living Materialsmentioning

confidence: 99%

“…The His-tags crosslink Ni-NTA-modified polyacrylamide to a hydrogel (see inset). The second module further contains an engineered caspase 3 variant (Casp3 OFF [ 187 ]) that is inducible by the 3C protease (3CPRO). Casp3 OFF is linked via a TCS and His-tag to Ni-NTA-polyacrylamide.…”

Section: Engineering Cells To Synthesize (Precursors Of) Non-living Materialsmentioning

confidence: 99%

Synthetic biology as driver for the biologization of materials sciences

Burgos-Morales

Gueye

Lacombe

et al. 2021

Materials Today Bio

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“…[ 42,43 ] Caspases have been engineered and utilized as versatile tools for synthetic circuits and information‐processing materials. [ 14,44 ]…”

Section: Introductionmentioning

confidence: 99%

Design of a Biohybrid Materials Circuit with Binary Decoder Functionality

Mohsenin,

Wagner,

Rosenblatt

et al. 2024

Advanced Materials

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Synthetic biology applies concepts from electrical engineering and information processing to endow cells with computational functionality. Transferring the underlying molecular components into materials and wiring them according to topologies inspired by electronic circuit boards has yielded materials systems that perform selected computational operations. However, the limited functionality of available building blocks is restricting the implementation of advanced information‐processing circuits into materials. Here, a set of protease‐based biohybrid modules the bioactivity of which can either be induced or inhibited is engineered. Guided by a quantitative mathematical model and following a design‐build‐test‐learn (DBTL) cycle, the modules are wired according to circuit topologies inspired by electronic signal decoders, a fundamental motif in information processing. A 2‐input/4‐output binary decoder for the detection of two small molecules in a material framework that can perform regulated outputs in form of distinct protease activities is designed. The here demonstrated smart material system is strongly modular and can be used for biomolecular information processing for example in advanced biosensing or drug delivery applications.

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“…[42,43] Caspases have been engineered and utilized as versatile tools for synthetic circuits and information-processing materials. [14,44] Design-build-test-learn (DBTL) iterative workflows in synthetic biology aim to accelerate the system development and optimization by reducing laboratory cost. DBTL gains enormous productivity when supported by in-depth data analysis and mathematical modelling.…”

Section: Introductionmentioning

confidence: 99%

“…[42,43] Caspases have been engineered and utilized as versatile tools for synthetic circuits and information-processing materials. [14,44]…”

Section: Introductionmentioning

confidence: 99%

Design of a Biohybrid Materials Circuit with Binary Decoder Functionality

Mohsenin

Wagner

Rosenblatt

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Synthetic biology applies concepts from electrical engineering and information processing to endow cells with computational functionality. Transferring the underlying molecular components into materials and wiring them according to topologies inspired by electronic circuit boards has yielded materials systems that perform selected computational operations. However, the limited functionality of available building blocks is restricting the implementation of advanced information-processing circuits into materials. Here, we engineer a set of protease-based biohybrid modules the bioactivity of which can either be induced or inhibited. Guided by a quantitative mathematical model and following a design-build-test-learn cycle, we wire the modules according to circuit topologies inspired by electronic signal decoders, a fundamental motif in information processing. We design a 2-input/4-output binary decoder for the detection of two small molecules in a material framework that could perform regulated outputs in form of distinct protease activities. The here demonstrated smart material system is strongly modular and could be used for biomolecular information processing for example in advanced biosensing or drug delivery applications.

show abstract

Design of a Human Rhinovirus-14 3C Protease-Inducible Caspase-3

Cited by 6 publications

References 31 publications

Synthetic biology as driver for the biologization of materials sciences

Synthetic biology as driver for the biologization of materials sciences

Design of a Biohybrid Materials Circuit with Binary Decoder Functionality

Design of a Biohybrid Materials Circuit with Binary Decoder Functionality

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