Design of a migration assay for human gingival fibroblasts on biodegradable magnesium surfaces

Amberg, R.; Elad, Akiva; Rothamel, Daniel; Fienitz, Tim; Szakács, Gábor; Heilmann, Silja; Witte, Frank

doi:10.1016/j.actbio.2018.08.034

Cited by 36 publications

(22 citation statements)

References 37 publications

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“…Furthermore, a previous study showed that Ag ions can replace Ag nanoparticles to achieve antimicrobial properties and maintain cell variability and proliferation (Mohiti-Asli et al, 2014). For Mg ions, the study showed that Mg primarily affected the adhesion and migration behavior of human gingival fibroblasts instead of proliferation (Amberg et al, 2018;Wang L. Y. et al, 2021). In the present study, no impaired variability or increased proliferation rate of the fibroblasts were observed.…”

Section: Cytocompatibility Evaluation Of the Fabricated Membranessupporting

confidence: 46%

RETRACTED: Metallic Ions Encapsulated in Electrospun Nanofiber for Antibacterial and Angiogenesis Function to Promote Wound Repair

Zhu

Cao

Chen

2021

Front. Cell Dev. Biol.

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Electrospun nanofiber is an attractive biomaterial for skin tissue engineering because it mimics the natural fibrous extracellular matrix structure and creates a physical structure suitable for skin tissue regeneration. However, endowing the nanofibrous membranes with antibacterial and angiogenesis functions needs to be explored. In the current study, we aimed to fabricate gelatin/polycaprolactone (GT/PCL) (GT/PCL-Ag-Mg) nanofibers loaded with silver (Ag) and magnesium (Mg) ions for antibacterial activity and pro-angiogenesis function for wound repair. The fabricated GT/PCL membranes had a nanofibrous structure with random arrangement and achieved sustained release of Ag and Mg ions. In vitro results indicated that the GT/PCL-Ag-Mg membranes presented satisfactory cytocompatibility with cell survival and proliferation. In addition, the membranes with Ag demonstrated good antibacterial capacity to both gram-positive and gram-negative bacteria, and the Mg released from the membranes promoted the tube formation of vascular endothelial cells. Furthermore, in vivo results demonstrated that the GT/PCL-Ag-Mg membrane presented an accelerated wound healing process compared with GT/PCL membranes incorporated with either Ag or Mg ions and pure GT/PCL alone. Superior epidermis formation, vascularization, and collagen deposition were also observed in GT/PCL-Ag-Mg membrane compared with the other membranes. In conclusion, a multifunctional GT/PCL-Ag-Mg membrane was fabricated with anti-infection and pro-angiogenesis functions, serving as a potential metallic ion-based therapeutic platform for applications in wound repair.

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Section: Cytocompatibility Evaluation Of the Fabricated Membranessupporting

confidence: 46%

RETRACTED: Metallic Ions Encapsulated in Electrospun Nanofiber for Antibacterial and Angiogenesis Function to Promote Wound Repair

Zhu

Cao

Chen

2021

Front. Cell Dev. Biol.

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“…Discs of JDBM and Ti with a diameter of 14 mm and height of 2.0 mm were ultrasonically cleaned by ethanol and acetone for 10 min and were further sterilized by exposure to ultraviolet light for another hour. JDBM samples were precorroded in Roswell Park Memorial Institute (RPMI) 1640 culture medium (Gibco, USA) supplemented with 10% inactivated fetal bovine serum (FBS) and 1% penicillin–streptomycin (PS) for 24 h (at 5% CO 2 and 37°C) to avoid the initial high corrosion and ensure cell adhesion (30). Ti discs and cell culture plates (CCPs) underwent the same pretreatment.…”

Section: Methodsmentioning

confidence: 99%

A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7–PI3K–AKT1 Signaling Axis

Jin

Chen

et al. 2019

Front. Immunol.

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Mg-based alloys might be ideal biomaterials in clinical applications owing to favorable mechanical properties, biodegradability, biocompatibility, and especially their anti-inflammatory properties. However, the precise signaling mechanism underlying the inhibition of inflammation by Mg-based alloys has not been elucidated. Here, we investigated the effects of a Mg-2.1Nd-0.2Zn-0.5Zr alloy (denoted as JDBM) on lipopolysaccharide (LPS)-induced macrophages. THP-1 cell-derived macrophages were cultured on JDBM, Ti−6Al−4V alloy (Ti), 15% extract of JDBM, and 7.5 mM of MgCl2 for 1 h before the addition of LPS for an indicated time; the experiments included negative and positive controls. Our results showed JDBM, extract, and MgCl2 could decrease LPS-induced tumor necrosis factor (TNF) and interleukin (IL)-6 expression. However, there were no morphologic changes in macrophages on Ti or JDBM. Mechanically, extract and MgCl2 downregulated the expression of toll-like receptor (TLR)-4 and MYD88 compared with the positive control and inhibited LPS-induced nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways by inactivation of the phosphorylation of IKK-α/β, IKβ-α, P65, P38, and JNK. Additionally, the LPS-induced reactive oxygen species (ROS) expression was also decreased by extract and MgCl2. Interestingly, the expression of LPS-induced TNF and IL-6 could be recovered by knocking down TRPM7 of macrophages, in the presence of extract or MgCl2. Mechanically, the activities of AKT and AKT1 were increased by extract or MgCl2 with LPS and were blocked by a PI3K inhibitor, whereas siRNA TRPM7 inhibited only AKT1. Together, our results demonstrated the degradation products of Mg-based alloy, especially magnesium, and resolved inflammation by activation of the TRPM7–PI3K–AKT1 signaling pathway, which may be a potential advantage or target to promote biodegradable Mg-based alloy applications.

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“…Cell migration was regulated by the local microenvironment, such as ECM rigidity, signaling factors and ligands on the ECM [43]. Although fast cell migration was not optimal for tissue repair, it could accelerate wound closure to reduce infections and the onset of pain and achieve a shortened healing period [44]. Cell scratch assay was applied to investigate the influence of rhCol III on HSF migration in vitro in this study.…”

Section: Cell Migrationmentioning

confidence: 99%

Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype

Wang

Wang³

et al. 2022

J Leather Sci Eng

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Collagen made a tremendous impact in the field of regenerative medicine as a bioactive material. For decades, collagen has been used not only as a scaffolding material but also as an active component in regulating cells' biological behavior and phenotype. However, animal-derived collagen as a major source suffered from problems of immunogenicity, risk of viral infection, and the unclear relationship between bioactive sequence and function. Recombinant humanized collagen (rhCol) provided alternatives for regenerative medicine with more controllable risks. However, the characterization of rhCol and the interaction between rhCol and cells still need further investigation, including cell behavior and phenotype. The current study preliminarily demonstrated that recombinant humanized collagen type III (rhCol III) conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine collagen. Furthermore, rhCol III could facilitate basal biological behaviors of human skin fibroblasts, such as adhesion, proliferation and migration. rhCol III was beneficial for some extracellular matrix-expressing cell phenotypes. The study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration. Graphical abstract

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Design of a migration assay for human gingival fibroblasts on biodegradable magnesium surfaces

Cited by 36 publications

References 37 publications

RETRACTED: Metallic Ions Encapsulated in Electrospun Nanofiber for Antibacterial and Angiogenesis Function to Promote Wound Repair

RETRACTED: Metallic Ions Encapsulated in Electrospun Nanofiber for Antibacterial and Angiogenesis Function to Promote Wound Repair

A Biodegradable Mg-Based Alloy Inhibited the Inflammatory Response of THP-1 Cell-Derived Macrophages Through the TRPM7–PI3K–AKT1 Signaling Axis

Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype

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