Design of a Multiepitope Vaccine against Chicken Anemia Virus Disease

Fatoba, Abiodun J.; Adeleke, Victoria T.; Maharaj, Leah; Okpeku, Moses; Adeniyi, Adebayo A.; Adeleke, Matthew Adekunle

doi:10.3390/v14071456

Cited by 12 publications

(5 citation statements)

References 47 publications

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“…The T-cell binding epitopes were analysed by screening short peptides that bind to human MHC molecule alleles from the selected fragments. Similar studies were conducted by other groups [ 43 , 44 ]. These researchers developed multiepitope vaccines targeting chicken anaemia virus and chicken Mycoplasma gallisepticum using the aforementioned screening methods.…”

Section: Discussionsupporting

confidence: 81%

Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Chen,

Su,

et al. 2023

Vet Res

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Clinical avian coccidiosis is typically caused by coinfection with several Eimeria species. Recombinant protein and DNA vaccines have shown promise in controlling coccidiosis. On this basis, DNA vaccines that encode multiple epitopes from different Eimeria species may provide broad protection against coinfections. In this study, we designed a fusion gene fragment, 14EGT, that contained concentrated T-cell epitopes from four common antigens of Eimeria species (14-3-3, elongation factor 2, glyceraldehyde-3-phosphate dehydrogenase, and transhydrogenase). The multiepitope DNA vaccine pVAX1-14EGT and recombinant protein vaccine pET-32a-14EGT (r14EGT) were then created based on the 14EGT fragment. Subsequently, cellular and humoral immune responses were measured in vaccinated chickens. Vaccination-challenge trials were also conducted, where the birds were vaccinated with the 14EGT preparations and later exposed to single or multiple Eimeria species to evaluate the protective efficacy of the vaccines. According to the results, vaccination with 14EGT preparations effectively increased the proportions of CD4+ and CD8+ T cells and the levels of Th1 and Th2 hallmark cytokines. The levels of serum IgG antibodies were also significantly increased. Animal vaccination trials revealed alleviated enteric lesions, weight loss, and oocyst output compared to those of the control groups. The preparations were found to be moderately effective against single Eimeria species, with the anticoccidial index (ACI) ranging from 160 to 180. However, after challenge with multiple Eimeria species, the protection provided by the 14EGT preparations was not satisfactory, with ACI values of 142.18 and 146.41. Collectively, the results suggest that a multiepitope vaccine that encodes the T-cell epitopes of common antigens derived from Eimeria parasites could be a potential and effective strategy to control avian coccidiosis.

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Section: Discussionsupporting

confidence: 81%

Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Chen,

Su,

et al. 2023

Vet Res

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“…The Protparam server ( https://web.expasy.org/protparam/ .) was utilized to calculate the physicochemical parameters of the proteins construct, including the instability index and grand average hydropathy (GRAVY) 47 . The number of transmembrane helixes in a protein is significant criterion for vaccine development, as they are relevant in the process to clone, express, and purify proteins.…”

Section: Methodsmentioning

confidence: 99%

Screening of immunogenic proteins and evaluation of vaccine candidates against Mycoplasma synoviae

Han

Chen

et al. 2023

npj Vaccines

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Mycoplasma synoviae (M. synoviae) is a serious avian pathogen that causes significant economic losses to chicken and turkey producers worldwide. The currently available live attenuated and inactivated vaccines provide limited protection. The objective of this study was to identify potential subunit vaccine candidates using immunoproteomics and reverse vaccinology analyses and to evaluate their preliminary protection. Twenty-four candidate antigens were identified, and five of them, namely RS01790 (a putative sugar ABC transporter lipoprotein), BMP (a substrate-binding protein of the BMP family ABC transporter), GrpE (a nucleotide exchange factor), RS00900 (a putative nuclease), and RS00275 (an uncharacterized protein), were selected to evaluate their immunogenicity and preliminary protection. The results showed that all five antigens had good immunogenicity, and they were localized on the M. synoviae cell membrane. The antigens induced specific humoral and cellular immune responses, and the vaccinated chickens exhibited significantly greater body weight gain and lower air sac lesion scores and tracheal mucosal thicknesses. Additionally, the vaccinated chickens had lower M. synoviae loads in throat swabs than non-vaccinated chickens. The protective effect of the RS01790, BMP, GrpE, and RS00900 vaccines was better than that of the RS00275 vaccine. In conclusion, our study demonstrates the potential of subunit vaccines as a new approach to developing M. synoviae vaccines, providing new ideas for controlling the spread of M. synoviae worldwide.

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“…The adhesion probability threshold was kept to 0.5, the immunogenicity threshold was kept to 90 to predict probable antigenic and non-antigenic proteins 48 . The Protparam server was used to calculate the physicochemical parameters of the proteins construct, which include the instability index and grand average hydropathy (GRAVY) 49 . The number of transmembrane helixes in a protein is significant criterion for vaccine development, as they are relevant in the process to clone, express, and purify proteins.…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Novel antigenic proteins of Mycoplasma synoviae as potential vaccine candidates

Shi

Han

et al. 2023

Preprint

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Mycoplasma synoviae (M. synoviae) is a serious avian pathogen causing severe economic losses to chicken and turkey producers worldwide. The currently available live attenuated vaccine and inactivated vaccine on the market elicit only limited protection. The aim of this study was to identify antigenic proteins of M. synoviae as potential subunit vaccine candidates. In immunoproteomics and reverse vaccinology analyses, a total 24 candidate antigens were picked out. Five candidate antigens were selected to evaluate immunogenicity and preliminary protection in a chicken model, including RS01790 (lipoprotein, putative sugar ABC transporter), BMP (BMP family ABC transporter substrate-binding protein), GrpE (nucleotide exchange factor), RS00900 (putative nuclease) and RS00275 (Uncharacterized protein). The results showed that the five antigens had good immunogenicity. They could be localized on the M. synoviae cell membrane and adhere to DF-1 cells using indirect immunofluorescence assay. They induced specific humoral and cellular immune responses. Vaccine efficacy was evaluated by observing weight gain, pathogen load, pathological changes. As a result, the vaccinated chickens revealed significantly higher body weight gain, with lower air sac lesion scores and tracheal mucosal thicknesses. The vaccinated chickens also showed lower M. synoviae loads in throat swabs than nonvaccinated chickens. The protective effect of BMP, GrpE and RS00900 vaccines is better than that of RS01790 and RS00275. In conclusion, our study demonstrates the potential of using subunit vaccine, providing new ideas for the development of M. synoviae vaccines. The vaccine candidates may contribute to the future development of therapeutic strategies to control the spread of M. synoviae worldwide.

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Design of a Multiepitope Vaccine against Chicken Anemia Virus Disease

Cited by 12 publications

References 47 publications

Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Protective efficacy of multiepitope vaccines constructed from common antigens of Eimeria species in chickens

Screening of immunogenic proteins and evaluation of vaccine candidates against Mycoplasma synoviae

Novel antigenic proteins of Mycoplasma synoviae as potential vaccine candidates

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