Design of a single incubation column radioimmunoassay

Verhoff, F. H.; Denning, C.E.; Boguslaski, Robert C.

doi:10.1021/ac50034a012

Anal. Chem.

1978

DOI: 10.1021/ac50034a012

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Design of a single incubation column radioimmunoassay

F. H. Verhoff¹,

C.E. Denning²,

Robert C. Boguslaski³

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1997

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References 19 publications

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Improved Ruggedness for Membrane-Based Amperometric Sensors Using a Pulsed Amperometric Method

Wang

Pardue

1997

Anal. Chem.

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This paper introduces a new approach to the use of membrane-based amperometric sensors which is expected to improve the ruggedness of these sensors significantly relative to the steady-state method in common use. In this new method, the fixed-voltage source used with the conventional steady-state method is replaced by a pulsed-voltage source. Unlike the fixed-source approach, which yields steady-state currents corresponding to large differences between analyte concentrations inside and outside the isolating membrane, the pulsed-source approach permits measurement of currents corresponding to near-equilibrium conditions between solutions inside and outside the membrane. Because the measured currents correspond to near-equilibrium conditions, results are expected to be virtually independent of variables that affect rates of mass transport to and across the membrane. The new approach is evaluated using the "oxygen electrode" as a model system. Results obtained using the new method are compared with results obtained using the conventional steady-state option as well as a coulometric approach described recently. The reproducibility of the pulsed amperometric approach and the scatter of data about least-squares calibration lines are an order of magnitude or more better than for the conventional steady-state option. As expected, the pulsed amperometric method is 40-100-fold less dependent on changes in membrane thickness, stirring rate, and temperature than the conventional steady-state option.

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Improved Ruggedness for Membrane-Based Amperometric Sensors Using a Pulsed Amperometric Method

Wang

Pardue

1997

Anal. Chem.

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Design of a single incubation column radioimmunoassay

Cited by 1 publication

References 19 publications

Improved Ruggedness for Membrane-Based Amperometric Sensors Using a Pulsed Amperometric Method

Improved Ruggedness for Membrane-Based Amperometric Sensors Using a Pulsed Amperometric Method

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