Design of an Electrochemical Biosensor for the Quantification of H₂O₂ for Application to Cardiovascular Diseases

Abstract

Electrochemical biosensors, which are fast and reliable analytical tools, have attracted particular attention in recent years for their ability to incorporate biomolecules into nanomaterial-designed electrodes. In this study, an electrochemical biosensor for the detection of hydrogen peroxide (H2O2) was developed using a multi-walled carbon nanotube (EPMWC) platform electrochemically modified with poly [Fe (III)-5-Aphen] and using an oxide-reductase enzyme as recognition agent. Amperometry was applied to obtain the calibration curves for H2O2 at the reduction process. The analytical parameters such as sensitivity, linear range, and low detection limits for H2O2 were obtained. A sensitivity of 26.51µA/mM, linear range from (0.4 to 4.5) H2O2 mM and a LOD of 0.761 µM. The advance of this measurement system is the first step in the development process of an electrochemical biosensor for cardiovascular diseases applications.

Advancements in electrochemical biosensing of cardiovascular disease biomarkers

“…The resulting change in current is then quantified and interpreted by the biosensor. 98,99 Crucially, the magnitude of this current is directly proportional to the concentration of the biomarker in the sample, thus allowing for quantification of the target biomarker. This type of biosensor typically features a bio-recognition element, such as an enzyme or an AB, immobilized on the electrode surface.…”