This study investigates the effects of emotional priming in virtual reality (VR) on time perception using a temporal bisection task and the potential effect of transcranial direct current stimulation (tDCS) over the right ventromedial prefrontal cortex (vmPFC) in hindering emotional processing. Fifty-three participants underwent active anodal or sham tDCS on the right vmPFC while exposed to neutral or fear-inducing VR videos. The participants then completed a temporal bisection task. The study measured arousal and valence through self-report questionnaires and psychophysiological measures (heart rate, heart rate variability, electrodermal activity). The results indicate that VR priming was effective in producing changes in arousal and valence, but this had no impact on time perception. Also, tDCS did not modulate the relationship between priming and time perception. These findings show the viability of using VR to generate emotional states, but these may not always produce changes in time perception. tDCS, as applied according to our protocol, also seemed unable to regulate fear processing.