Design of an interdigitated microelectrode biosensor using a-SiC:H surface to capture E. coli

Herrera-Celis, Jośe; Reyes-Betanzo, C.; Orduña-Díaz, A.

doi:10.1109/sbmicro.2015.7298142

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…These findings had already been observed by simulations in CoventorWare software using neutral PBS as the medium. 34 According to these simulations, the shifting of the impedance spectrum to the right is due to the presence of bacteria on microelectrodes.…”

Section: Effects Of the Conductivity Of The Medium And Bacteria Onto Mi-mentioning

confidence: 90%

“…The a-Si x C 1-x :H layer on Ti was doped to reduce its impact on the PIMA sensitivity. 34 Thus, the whole surface on the array is available for capturing analytes once the surface of the PIMA is biofunctionalized. There are four geometric parameters to set: finger spacing (s), finger width (w), finger length (p), and sensing area (A s ).…”

Section: Design Fabrication and Characterization Of The Pimasmentioning

confidence: 99%

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Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection

Herrera-Celis

Reyes-Betanzo

Torres‐Jácome

et al. 2017

J. Electrochem. Soc.

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Fabrication and testing of interdigitated microelectrode arrays whose structure includes non-cytotoxic hydrogenated amorphous silicon-carbon alloy (a-Si x C 1-x :H) as the surface to be biofunctionalized for capturing enteropathogenic Escherichia coli (E. coli, EPEC) are presented. a-Si x C 1-x :H films were obtained by enhanced chemical vapor deposition (PECVD). The extract method was used to assess the cytotoxicity of the films. The design of the PIMAs includes two layers of a-Si x C 1-x :H, one intrinsic layer deposited onto silicon dioxide (SiO 2 ) before evaporating titanium (Ti), and one doped layer deposited onto the Ti-microelectrodes. Electrical impedance spectroscopy (EIS) was used to know the effects of the biofunctionalization layer, conductivity of the medium and any capture of bacteria by antibodies on the microelectrodes. According to the results, the high hydrogen dilution contributes to low incorporation of CH n groups improving the non cytotoxicity of the films, and the capture of bacteria on the microelectrodes improves the sensitivity. It manifests itself as a shift of the low cutoff frequency (F low ) of the impedance spectrum to the right, allowing the device to sense at frequencies lower than F low . A percentage change in impedance of 1600% at 100 Hz was obtained after 5 minutes in contact with medium with EPEC concentration of 8.5 × 10 8 CFU/mL.

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Section: Effects Of the Conductivity Of The Medium And Bacteria Onto Mi-mentioning

confidence: 90%

Section: Design Fabrication and Characterization Of The Pimasmentioning

confidence: 99%

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection

Herrera-Celis

Reyes-Betanzo

Torres‐Jácome

et al. 2017

J. Electrochem. Soc.

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“…Returning to the results in Table 2, the biosensor sensitivity increases when a microfluidic channel is integrated to confine the bacterial sample to the area between microelectrodes. Although the use of the area on the microelectrodes would allow to house a greater number of CFUs, the contribution to the capacitance of one bacterium on microelectrodes is different to that obtained by one bacterium between microelectrodes [15], which would add uncertainty to the measurement. Furthermore, if the microfluidic channel were integrated on microelectrodes, the parasitic capacitances would give rise to a poor performance of the device.…”

Section: Discussionmentioning

confidence: 99%

Design of a Biosensor Based on Interdigitated Microelectrodes with Detection Zone Controlled by an Integrated Microfluidic Channel

Zazueta-Gambino

Reyes-Betanzo²,

Herrera-Celis³

2020

JICS

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The design and simulation of a biosensor based on interdigitated microelectrodes for bacteria detection is presented. The biosensor includes a microchannel to ensure the flow of the sample through the space between microelectrodes, where the surface is biofunctionalized with antibodies to capture the bacteria. The design was built on COMSOL Multiphysics® software. The effects of the microelectrode thickness and the channel depth on the biosensor sensitivity were studied by simulation. There is a specific microelectrode thickness at which the sensitivity is maximum for Escherichia coli. The microchannel depth affects the sensitivity of the device when it is below 10 μm, approximately. The sensitivity increases when the biosensor is made with low-permittivity materials. A maximum percentage change in capacitance of around 46% was obtained by covering the total sensing area with bacteria.

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“…The IMA used in this work includes a-SiC:H on and between interdigitated microelectrodes (IM) as surface to be biofunctionalized [9]. This characteristic allows to capture bacteria of E. coli on whole surface of the array.…”

Section: B Interdigitated Microelectrode Arraymentioning

confidence: 99%

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

Herrera-Celis

Reyes-Betanzo

Orduña-Díaz

et al. 2017

VII Latin American Congress on Biomedical Engineering CLAIB 2016, Bucaramanga, Santander, Colombia, October 26th -28th, 2016

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Abstract-A biofunctionalization process of a-SiC:H surfaces has been applied to an interdigitated microelectrode array (IMA) whose microelectrodes are covered by this material. The biofunctionalization process has been monitored stage by stage using Fourier transform infrared spectroscopy (FTIR), while its effects on the electrical behavior of the IMA were recorded in electrical impedance spectra. The process involves hydroxylation, silanization, generation of aldehyde groups, binding via protein A, immobilization of anti-Escherichia coli polyclonal antibodies, entrapping and detection of enterotoxigenic Escherichia coli (ETEC) in Luria-Bertani medium. The FTIR spectra confirm the success of the process. Regarding the performance of the IMA, although the detection of ETEC is successful and its percentage change in impedance reaches a value of 133.37% to 10 7 CFU/mL, some considerations may be taken into account to improve the sensitivity of the IMA by mean of the optimization of both the IMA design and the biofunctionalization process.Keywords-Hydrogenated amorphous silicon carbide, biofunctionalization process, enterotoxigenic Escherichia coli, Fourier transform infrared spectroscopy, electrical impedance spectroscopy.

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Design of an interdigitated microelectrode biosensor using a-SiC:H surface to capture E. coli

Cited by 4 publications

References 17 publications

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection

Design of a Biosensor Based on Interdigitated Microelectrodes with Detection Zone Controlled by an Integrated Microfluidic Channel

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

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Design of an interdigitated microelectrode biosensor using a-SiC:H surface to capture E. coli

Cited by 4 publications

References 17 publications

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-SixC1-x:H forE. coliDetection

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-SixC1-x:H forE. coliDetection

Design of a Biosensor Based on Interdigitated Microelectrodes with Detection Zone Controlled by an Integrated Microfluidic Channel

Biofunctionalization Process of a-SiC:H Surfaces Applied to an Interdigitated Microelectrode Array to Detect Enterotoxigenic Escherichia coli

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Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection

Interdigitated Microelectrode Arrays Based on Non-Cytotoxica-Si_xC_1-x:H forE. coliDetection