Design of an optical nanobiosensor for detection of Legionella pneumophila in water samples

Abstract

Background and Objectives: Legionella spp. is a causative agent of Legionnaires' disease that creates public health prob- lems. Isolation of these bacteria from water sources is essential to identify outbreak origins and prevent disease. Diagnostic biosensors for water quality control to protect consumers from water-borne infections can predict many outbreaks. Gold nanoparticles conjugated probes are a new generation of diagnostic tools. In this study, an opticalnano biosensor was de- signed and characterized to detect Legionella pneumophila in water samples rapidly.
 Materials and Methods: Thiolated probes designed for the mip gene were attached to gold nanoparticles and then water samples containing Legionella pneumophila were examined.
 Results: The limit of detection for PCR and biosensor was 104 and 103 copy numbers/µl, respectively. Biosensor sensitivity and PCR were reported to be 90%(18 out of 20) and 85% (17 out of 20), respectively. Specificity 100% has been reported for both methods.
 Conclusion: According to the obtained results, this method has the potential to diagnose L. pneumophila with high sensi- tivity and specificity. This systemcan be employed as a practical tool for rapid, accurate, high-sensitivity, and acceptable detection of Legionella pneumophila in contaminated water, which is cost-effective in terms of cost and time.