Design of artificial extracellular matrices for tissue engineering

Kim, Byung Soo; Park, In Kyu; Hoshiba, Takashi; Jiang, Hu‐Lin; Choi, Yun‐Jaie; Akaike, Toshihiro; Cho, Chong‐Su

doi:10.1016/j.progpolymsci.2010.10.001

Cited by 265 publications

(167 citation statements)

References 245 publications

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“…Three-dimensional culture systems have been widely used in endothelial cell functional investigation, in which endothelial cells have shown differential responses to stimuli compared with conventional 2D culture systems [57,58]. Increasing evidence suggests that 3D culture systems have morphologic and physiologic similarities to physiological environments [59][60][61][62]. The activation of the cytoskeletal contractile machinery is critical for cell motility [63].…”

Section: Discussionmentioning

confidence: 99%

The Role of MCPIP1 in Ischemia/Reperfusion Injury-Induced HUVEC Migration and Apoptosis

Zhu

Yao

Hu³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Monocyte chemotactic protein-induced protein 1 (MCPIP1) plays a crucial role in various cellular processes, including neurogenesis. However, the relationship between MCPIP1 and myocardial ischemia/reperfusion (I/R) injury remained illdefined. In this study, we explored whether the I/R-mediated up-regulation of MCPIP1 is critical in the modulation of both cell migration and apoptosis in human umbilical vein endothelial cells (HUVECs). Methods: Using Western blot analysis and quantitative real-time PCR, the protein expression and mRNA transcription, respectively, of MCPIP1 was detected in HUVECs. To investigate cell migration, an in vitro scratch assay and a nested matrix model were applied. Results: I/R increased the expression of MCPIP1 via the activation of the mitogen-activated protein kinase (MAPK) and PI3K/Akt pathways. I/R increased migration and apoptosis of HUVECs, which were significantly inhibited by MCPIP1 siRNA. Conclusion: These findings suggest that I/R-mediated up-regulation of MCPIP1 regulates migration and apoptosis in HUVECs. Understanding the regulation of MCPIP1 expression and function may aid in the development of an adjunct therapeutic strategy in the treatment of individuals with I/R injury.

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Section: Discussionmentioning

confidence: 99%

The Role of MCPIP1 in Ischemia/Reperfusion Injury-Induced HUVEC Migration and Apoptosis

Zhu

Yao

Hu³

et al. 2015

Cell Physiol Biochem

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“…The process of adhesion regulates cell shape and biomechanics and is required for a variety of other cellular processes including proliferation, diferentiation, migration, and invasion [7].…”

Section: Adherence To Plastic In Standard Culture Conditionsmentioning

confidence: 99%

Physical versus Immunological Purification of Mesenchymal Stem Cells

Mehanna¹

2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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A prerequisite before dealing with any cell type is to identify it and isolate it from the heterogeneous cell population that it belongs to. Mesenchymal stem cells (MSC) can be found in nearly all tissues and are mostly located in perivascular niches.MSC isolated from Bone marrow, adipose tissue, peripheral blood and diferent organs had shown promising potential for proliferation and diferentiation into diferent cell types. They exhibit plastic-adherence under standard culture conditions, and this physical method of isolation is widely used as it is the most economic method and yet reveals relatively puriied populations of cells after 3 or 4 passages. The complete puriication still needs a speciic call to diferent MSCs subsets. This could be achieved by immunological sorting, which depends on identifying cell marker(s) of such cells. Selecting these cells using antibodies against their speciic markers then sorting the cells either by Magnetic or lorescence based techniques named Magnetic Activated Cell Sorting (MACS) or Florescence Activated Cell Sorting (FACS) respectively is the principle of such puriication techniques.The aim of this chapter is to thoroughly deine MSCs and compare between the diferent available methods for their puriication

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“…Surface functionalization of membranes with biomolecules is performed using biological species that usually have some of the specific functions of an organ (e.g., heparin which inhibits internal coagulation or urokinase for kidney function) [24]. Other parameters to be taken into account refer to the proliferation of cells on the material, its mechanical strength, and chemical interaction with species dispersed or dissolved in biological fluids coming in contact with the material [25].…”

Section: Membranes For Artificial Organsmentioning

confidence: 99%