2017
DOI: 10.1021/acs.cgd.7b01398
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Design of Bulk Protein Crystallization Based on Phase Diagrams Accounting for the Presence of Interfacial Water

Abstract: An effective procedure for design of protein crystallization was proposed, in which binary and ternary phase diagrams were exploited to evaluate the process performance. A method for the construction of phase diagrams that accounted for the presence of water associated with the protein crystalline phase (interfacial water) was described. The model system for the study included ovalbumin and lysozyme, which were crystallized from aqueous solutions of ammonium sulfate. The design procedure consisted of the deter… Show more

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Cited by 7 publications
(10 citation statements)
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“…The difference in the equilibrium concentrations for the protein solutions with the same initial salt content (pairs of the curves 1,2; 3,4; 5,6 in Figure ) can be attributed to water uptake by protein crystals in the solid phase . The depletion of water in the solution induces a change in the salt concentration and, consequently, in the protein solubility.…”
Section: Resultsmentioning
confidence: 98%
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“…The difference in the equilibrium concentrations for the protein solutions with the same initial salt content (pairs of the curves 1,2; 3,4; 5,6 in Figure ) can be attributed to water uptake by protein crystals in the solid phase . The depletion of water in the solution induces a change in the salt concentration and, consequently, in the protein solubility.…”
Section: Resultsmentioning
confidence: 98%
“…The protein solubility and the operating window for the crystallization of OVA were determined in our previous work . The position of the window is illustrated on the binary phase diagram as a function of the protein mass fraction versus the salt mass fraction, which is presented in Figure .…”
Section: Resultsmentioning
confidence: 99%
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