“…To understand the molecular mechanisms of transport it is of great interest to identify and to analyze how much charge is moved through the protein in each of the reaction steps [1]. Various techniques are applied to investigate the electrogenicity of Na transport: voltage jump experiments with giant patch or whole cell patch of myocardial cells [4,5], charge pulse experiments on membrane fragments adsorbed to planar lipid bilayers [6,7] or, very recently, adsorbed to solid supported bilayers [8], and a £uorescence technique applying an electrochromic styryl dye, RH421, which detects charge movements within the membrane dielectric [9,10]. The latter method provides indirect access to the so-called electrogenicity as discussed elsewhere [1,6,10].…”