Purpose: It is now clear that the COVID-19 viruses can be transferred via airborne transmission. The objective of this study was to attempt the design and fabrication of an AMBU ventilator with a negative pressure headbox linked to a negative pressure transporting capsule, which could provide a low-cost construction, flexible usage unit, and also airborne prevention that could be manufactured without a high level of technology.Method: The machine consists of an automated AMBU bag ventilator, a negative pressure headbox, and a transporting capsule. The function and working duration of each component were tested.Results: The two main settings of the ventilator include an active mode that can be set at the time range of 0 s–9 h 59 min 59 s and a resting mode, which could work continuously for 24 h. The blower motor and battery system, which were used to power the ventilator, create negative air pressure within the headbox, and the transporting capsule, could run for at least 2 h without being recharged. The transporting capsule was able to create an air change rate of 21.76 ACH with-10 Pa internal pressure.Conclusion: This automated AMBU ventilator allowed flow rate, rhythm, and volume of oxygen to be set. The hazardous expired air was treated by a HEPA filter. The patient’s transporting capsule is of a compact size and incorporates the air treatment systems. Further development of this machine should focus on how to link seamlessly with imaging technology, to verify standardization, to test using human subjects, and then to be the commercialized.