Design of experiment techniques for the optimization of chromatographic analysis conditions: A review

Abstract

Design of experiment (DoE) techniques have been widely used in the field of chromatographic parameters optimization as a valuable tool. A systematic literature review of the available DoE techniques applied to the development of a chromatographic analysis method is presented in this paper. First, the most common available designs and the implementation steps of DoE are comprehensively introduced. Then the studies in recent 10 years for the application of DoE techniques in various chromatographic techniques are discussed, such as capillary electrophoresis, liquid chromatography, gas chromatography, thin‐layer chromatography, and high‐speed countercurrent chromatography. Current problems and future outlooks are finally given to provide a certain inspiration of research in the application of DoE techniques to the different chromatographic techniques field. This review contributes to a better understanding of the DoE techniques for the efficient optimization of chromatographic analysis conditions, especially for the analysis of complex systems, such as multicomponent drugs and natural products.

Strategies for capillary electrophoresis: Method development and validation for pharmaceutical and biological applications—Updated and completely revised edition

“…A design of experiments (DoE) approach is great for identifying a suitable analytical method, as later described in Ref. [3].…”

“…Under these conditions, it is important to carefully monitor the separation current, as Joule heating could become problematic and favor the development of bubbles, especially if a volatile modifier (such as methanol) is used. Optimization of these variables can be accomplished by a number of strategies, including univariate modes [42], multivariate analysis [43,44], a linear decision tree [45], DoE [3], or even DFT calculations [46].…”

Unlocking Therapeutic Potential: Comprehensive Extraction, Profiling, and Pharmacological Evaluation of Bioactive Compounds from Eclipta alba (L.) Hassk. for Dermatological Applications

“…Thin layer chromatography (TLC) is a widely applied chromatographic method for the separation and identification of bioactive substances by comparing the retention factor (Rf) of these bioactive substances [ 103 ]. Previous studies conducted using TLC and HPTLC are summarized in Table 4 .…”