Design of Experimentation to Systematically Determine the Interaction Between Electrospinning Variables and to Optimize the Fiber Diameter of Electrospun Poly (D,l-Lactide-Co-Glycolide) Scaffolds for Tissue Engineered Constructs

Castillo, Yvette S.

doi:10.15368/theses.2012.97

Cited by 5 publications

(12 citation statements)

References 151 publications

(128 reference statements)

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“…[24] It should be noted that there is also an optimum concentration under which beads or nanoparticles might be formed and for higher concentrations the solution is not spinnable. [27] Similar diameter sizes were obtained for polyurethane-dextran [28] and whey protein [29] nanofibres.…”

Section: Electrospinning Of Vitamin E-loaded Dextran Solutionssupporting

confidence: 62%

“…Thus there is an ideal applied voltage that results in a minimum fibre diameter depending on the polymer and interacting parameters. [27] Under a fixed applied voltage, higher amounts of polymer in the solution led to thinner and more uniform fibres. Changing the polymer concentration can vary the solution viscosity.…”

Section: Electrospinning Of Vitamin E-loaded Dextran Solutionsmentioning

confidence: 99%

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Characteristics of vitamin E-loaded nanofibres from dextran

Fathi

Nasrabadi

Varshosaz

2017

International Journal of Food Properties

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In this research, dextran nanofibres were produced as novel carriers for entrapment of vitamin E. Morphological studies indicated that developed fine fibres were in the nano range without spherical beads. Thermal behaviour, chemical structure, and crystalline structure of vitamin E-loaded nanofibres were analysed by differential scanning calorimetry, Fourier transform infrared spectroscopy, and X-ray diffraction which showed that the vitamin was well incorporated within the amorphous structure of fibres without chemical interactions. Nanofibres were used for fortification of cheese. Sensory analysis of the fortified product was performed which showed better acceptability and texture of cheese containing nanofibres in comparison to blank and direct fortified samples. Nanofibres showed the capability of holding water and thus increasing cheese firmness. The release of vitamin E in gastrointestinal media showed that about 14% of vitamin could be released in gastric media and about 30% released in intestinal media. Finally, the results revealed that electrospinning can be used for production of dextran ultrathin fibres to entrap hydrophobic compounds with high potential for design of novel functional foods. ARTICLE HISTORY

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Section: Electrospinning Of Vitamin E-loaded Dextran Solutionssupporting

confidence: 62%

Section: Electrospinning Of Vitamin E-loaded Dextran Solutionsmentioning

confidence: 99%

Characteristics of vitamin E-loaded nanofibres from dextran

Fathi

Nasrabadi

Varshosaz

2017

International Journal of Food Properties

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“…Scaffolds have three main functions in the tissue engineering process: (1) provide the overall shape of the construct, (2) facilitate the exchange of molecular and mechanical signals, and (3) support and optimize cellular functions 9 . The Cal Poly lab utilizes PLGA scaffolds to carry out these three functions in BVM cultivation 10,11 . These functions are determined by the characteristics of the scaffolds.…”

Section: Scaffoldsmentioning

confidence: 99%

“…This inversely proportional relationship was established in previous work by Cal Poly students and is defined at an optimal distance for the current electrospinning setup 10,11 . Research also indicates that increasing the voltage can reduce fiber diameter and uniform fiber diameters are reached at higher voltages 64,75 .…”

Section: Introductionmentioning

confidence: 97%

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Characterizing the Reproducibility of the Properties of Electrospun Poly(d,l-Lactide-Co-Glycolide) Scaffolds for Tissue-Engineered Blood Vessel Mimics

Pipes¹

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A design of experiments approach to identify the influencing parameters that determine poly-D,L-lactic acid (PDLLA) electrospun scaffold morphologies

et al. 2017

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Electrospun fibrous materials have increasing applications in regenerative medicine due to the similarity of fibre constructs to the morphology of certain extracellular matrices. Although experimentally the electrospinning method is relatively simple, at the theoretical level the interactions between process parameters and their influence on the fibre morphology is not yet fully understood. Here, we hypothesised that a design of experiments (DoE) model could determine combinations of process parameters that result in significant effects on poly-D,L-lactic acid (PDLLA) fibre morphology. The process parameters used in this study were applied voltage, needle-to-collector distance, flow rate and polymer concentration. Data obtained for mean fibre diameter, standard deviation (SD) of the fibre diameter (measure of fibre morphology) and presence of 'beading' on the fibres (beads per μm) were evaluated as a measure of PDLLA fibre morphology. Uniform fibres occurred at SDs of ≤500 nm, 'beads-on-string' morphologies were apparent between ±500 and 1300 nm and large beads were observed at ±1300-1800 nm respectively. Mean fibre diameter was significantly influenced by the applied voltage and interaction between flow rate and polymer concentration. Fibre morphology was mainly influenced by the polymer concentration, while bead distribution was significantly influenced by the polymer concentration as well as the flow rate. The resultant DoE model regression equations were tested and considered suitable for the prediction of parameters combinations needed for desired PDLLA fibre diameter and additionally provided information regarding the expected fibre morphology.

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Design of Experimentation to Systematically Determine the Interaction Between Electrospinning Variables and to Optimize the Fiber Diameter of Electrospun Poly (D,l-Lactide-Co-Glycolide) Scaffolds for Tissue Engineered Constructs

Cited by 5 publications

References 151 publications

Characteristics of vitamin E-loaded nanofibres from dextran

Characteristics of vitamin E-loaded nanofibres from dextran

Characterizing the Reproducibility of the Properties of Electrospun Poly(d,l-Lactide-Co-Glycolide) Scaffolds for Tissue-Engineered Blood Vessel Mimics

A design of experiments approach to identify the influencing parameters that determine poly-D,L-lactic acid (PDLLA) electrospun scaffold morphologies

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