Design of highly sensitive phosphorescence sensor for determination of procaterol hydrochloride based on inhibition of KClO₃ oxidation fluorescein isothiocyanate

Abstract

Procaterol hydrochloride (Prh) can inhibit KClO3 oxidation of fluorescein isothiocyanate (FITC) to form a non-phosphorescent compound, which causes room temperature phosphorescence (RTP) of FITC in the system to enhance sharply the linear relationship between ∆Ip and the Prh content. Thus, a rapid response and highly sensitive phosphorescence sensor for the determination of Prh has been developed based on the inhibiting effect of Prh on KClO3 oxidation of FITC. This simple, high sensitivity (detection limit (LD) calculated by 3Sb /k was 0.019 fg/spot, sample volume 0.40 µl, corresponding concentration 4.8 × 10(-14) g ml(-1) ) and selective sensor with a wide linear range (0.080-11.20 g/spot) has been applied to detect Prh in blood samples, and the results were consistent with those obtained by high-performance liquid chromatography (HPLC). Simultaneously, the mechanism of the phosphorescence sensor for the detection of Prh was also investigated using infrared spectroscopy.

Exploring the electrochemiluminescent behavior of procaterol hydrochloride in the presence of Ru(bpy)32+ and its analytical application in pharmaceutical preparation

“…Table displays the average results of seven replicate measurements expressed at a 95% level of confidence obtained for each sample solution using the newly proposed ECL method. The reliability of the proposed technique was evaluated statistically compared with the results of the HPLC method . As shown in Table , the results obtained using the proposed ECL method are consistent with those obtained using HPLC.…”

“…Table shows the comparison between the analytical performance of the present method and previous literature methods for the determination of ProH. From Table , we found that the proposed ECL method gave a LOD of 0.011 μM, which was better than the LOD of CE‐CD (0.06 μM), CE‐ECL (0.12 μM) and FIA‐CL (0.018 μM) methods, and much higher than that obtained with GC‐ESI‐MS (1.5 × 10 −5 μM) and phosphorescence (1.4 × 10 −5 μM) . However, compared with the GC‐ESI‐MS and phosphorescence methods mentioned above, the new ECL method was much simpler in apparatus and operation.…”

“…05), adding water to dissolve and adjusting with phosphate/methanol (78 : 22), detected at 259 nm. Quantitative analysis: the external standard method …”

“…Consequently, in order to address the potential prevention of adverse effects, a simple, rapid and sensitive method for the determination of ProH is of great importance in pharmacokinetic and clinical studies. To date, several methods have been developed for monitoring ProH in biological systems and pharmaceutical preparations, such as gas chromatograph/electron impact ionization mass spectrometry (GC‐ESI‐MS), capillary electrophoresis (CE), inhibited phosphorescence and flow‐injection chemiluminescence (FI‐CL)…”

Application of a fluorescent biosensor based‐on magneto‐γ‐Fe2O3–methyldopa nanoparticles for adsorption of human serum albumin

“…At present, there are many reports for the determination of biomolecules, such as IgE , myoglobin , dopamine , C‐reactive protein , procaterol hydrochloride , cefditoren pivoxil , nicotinamide by using analytical methods. Quantification of albumin is clinically important, as it has been found to be an effective prognostic indicator.…”