Design of Highly Stable Echogenic Microbubbles through Controlled Assembly of Their Hydrophobin Shell

Gazzera, Lara; Milani, Roberto; Pirrie, Lisa; Schmutz, Marc; Blanck, Christian; Resnati, Giuseppe; Metrangolo, Pierangelo; Krafft, Marie Pierre

doi:10.1002/anie.201603706

Cited by 26 publications

(20 citation statements)

References 24 publications

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“…First, the adsorption process is accelerated, and second, the equilibrium interfacial tensions are significantly lowered. We have reported similar effects of the fluorocarbon gas on the adsorption of a range of molecules, including phospholipids [23], polymers [7], proteins [24], biomarkers [25], and of CeO2 nanoparticles [26]. But the most important finding here is that the fluorocarbon gas affects the adsorption of IONPs differently, depending on whether the dendron carries a fluorinated end group or not.…”

Section: Adsorption Kinetics Of Dendronized Nanoparticles At the Gas/supporting

confidence: 50%

Microbubbles Decorated with Dendronized Magnetic Nanoparticles for Biomedical Imaging. Effective Stabilization via Fluorous Interactions

Shi¹,

Wallyn²,

Nguyen³

et al. 2019

Preprint

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Dendrons fitted with three oligoethylene glycol (OEG) chains, one of which carrying a fluorinated or hydrogenated end group, and bearing a bisphosphonate polar head (C n X2 n +1OEG8Den, X = F or H; n= 2 or 4) were synthesized and grafted on the surface of iron oxide nanoparticles (IONPs) for microbubble-mediated imaging and therapeutic purposes. The size and stability of the dendronized IONPs (IONP@C n X2 n +1OEG8Den) in aqueous dispersions were monitored by dynamic light scattering. Investigation of the spontaneous adsorption of IONP@C n X2 n +1OEG8Den at the interface between air - or air saturated with perfluorohexane - and an aqueous phase establishes that exposure to the fluorocarbon gas markedly increases the rate of adsorption of the dendronized IONPs to the gas/water interface and decreases the equilibrium interfacial tension. This suggests that fluorous interactions are at play between the supernatant fluorocarbon gas and the fluorinated end groups of the dendrons. Furthermore, small, stable perfluorohexane-stabilized microbubbles (MBs) with a dipalmitoylphosphatidylcholine (DPPC) shell that incorporates IONP@C n X2 n +1OEG8Den (DPPC/Fe molar ratio 28:1) were prepared and characterized using both optical microscopy and an acoustical method of size determination. The dendrons fitted with fluorinated end groups lead to smaller and more stable MBs than those fitted with hydrogenated groups. The most effective result is already obtained with C2F5, for which MBs, ~1.0mm in radius, reach a half-life of ~6.0 h. An atomic force microscopy investigation of spin-coated mixed films of DPPC/IONP@C2X5OEG8Den combinations (molar ratio 28:1) shows that the IONPs grafted with the fluorinated dendrons are located within the phospholipid film, while those grafted with the hydrocarbon dendrons are completely absent from the phospholipid film.

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Section: Adsorption Kinetics Of Dendronized Nanoparticles At the Gas/supporting

confidence: 50%

Microbubbles Decorated with Dendronized Magnetic Nanoparticles for Biomedical Imaging. Effective Stabilization via Fluorous Interactions

Shi¹,

Wallyn²,

Nguyen³

et al. 2019

Preprint

Self Cite

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“…The good surface activity and exceptionally high surface elasticity of HFBs makes them the most surface-active proteins currently known, which promoted their successful Brought to you by | MIT Libraries Authenticated Download Date | 5/12/18 9:00 AM exploitation in several different fields. Some of the uses proposed for HFBs include stabilization of foams and emulsions in the food industry [16], biotechnological applications such as drug delivery [17,18], biomedical imaging [19] and coatings for biomedical devices [20], but also use as dispersing agents [21,22] and intermediates for surface immobilization of proteins [23,24] and polymers [25]. The ability of HFBs to self-assemble at oil-water interfaces and stabilize oil droplets makes them potential candidates for a more efficient and greener EOR strategy, also in consideration of the fact that the potential for large scale production of these proteins has been shown in industrial operations for both classes of HFBs [14,16].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the potential of natural surfactants in the petroleum industry: the case of hydrophobins

Blešić

Dichiarante

Milani

et al. 2017

Pure and Applied Chemistry

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Enhancing oil recovery from currently available reservoirs is a major issue for petroleum companies. Among the possible strategies towards this, chemical flooding through injection of surfactants into the wells seems to be particularly promising, thanks to their ability to reduce oil/water interfacial tension that promotes oil mobilization. Environmental concerns about the use of synthetic surfactants led to a growing interest in their replacement with surfactants of biological origin, such as lipopeptides and glycolipids produced by several microorganisms. Hydrophobins are small amphiphilic proteins produced by filamentous fungi with high surface activity and good emulsification properties, and may represent a novel sustainable tool for this purpose. We report here a thorough study of their stability and emulsifying performance towards a model hydrocarbon mixture, in conditions that mimic those of real oil reservoirs (high salinity and high temperature). Due to the moderate interfacial tension reduction induced in such conditions, the application of hydrophobins in enhanced oil recovery techniques does not appear feasible at the moment, at least in absence of co-surfactants. On the other hand, the obtained results showed the potential of hydrophobins in promoting the formation of a gel-like emulsion 'barrier' at the oil/water interface.

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“…[1,2] Lipid-based MBs [1][2][3] are the most versatile and have demonstrated exciting applications as ultrasound theranostic agents,c ancer therapeutics,and more recently,i ntravenous oxygen carriers. [4] However,l ipid systems often lack the stability needed for many practical uses and tremendous efforts have been made to identify alternative shell materials, [5] forexample,proteins [6] and polyvinyl alcohol. [7] Notably,m icro-and nanoparticle-stabilized (Pickering-type) MBs have been developed that exhibit superior stability.…”

mentioning

confidence: 99%

Interfacial Nanoprecipitation toward Stable and Responsive Microbubbles and Their Use as a Resuscitative Fluid

et al. 2018

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A new approach has been developed to prepare stable microbubbles (MBs) by interfacial nanoprecipitation of bioabsorbable polymers at air/liquid interfaces. This facile method offers robust control over the morphology and chemophysical properties of MBs by simple chemical modifications. This approach is amenable to large-scale manufacturing, and is useful to develop functional MBs for advanced biomedical applications. To demonstrate this, a MB-based intravenous oxygen carrier was created that undergoes pH-triggered self-elimination. Intravenous injection of previous MBs increased the risk of pulmonary vascular obstruction. However, we show, for the first time, that our current design is superior, as they 1) yielded no evidence of acute risks in rodents, and 2) improved the survival in a disease model of asphyxial cardiac arrest (from 0 to 100 %), a condition that affects more than 100 000 in-hospital patients, and carries a mortality of about 90 %.

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Design of Highly Stable Echogenic Microbubbles through Controlled Assembly of Their Hydrophobin Shell

Cited by 26 publications

References 24 publications

Microbubbles Decorated with Dendronized Magnetic Nanoparticles for Biomedical Imaging. Effective Stabilization via Fluorous Interactions

Microbubbles Decorated with Dendronized Magnetic Nanoparticles for Biomedical Imaging. Effective Stabilization via Fluorous Interactions

Evaluating the potential of natural surfactants in the petroleum industry: the case of hydrophobins

Interfacial Nanoprecipitation toward Stable and Responsive Microbubbles and Their Use as a Resuscitative Fluid

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