In the context of thermal fusion tubing operations in biopharmaceutical manufacturing, we are currently researching an instrument designed to autonomously carry out tubing connections, thereby reducing manual intervention and effectively preventing contamination. The device is centered around the STM32 as its core control chip, featuring a multi-axis control module and motor speed regulation to drive the moving components. Additionally, we have implemented a temperature control module utilizing a fuzzy self-tuning PID control method for more precise temperature regulation of the blades. To enhance user experience, a human-machine interface has been incorporated, supporting functions such as three-level authorization and parameter settings. Following the completion of device assembly, performance testing was conducted, revealing a maximum temperature deviation of 2.3°C. The sealing and sterility criteria are in compliance with the design requirements, demonstrating that the device meets the demands for automated thermal fusion tubing connections in aseptic tubing transfer systems.