Design of Novel Biocompatible and Green Aqueous two-Phase Systems containing Cholinium L-alaninate ionic liquid and polyethylene glycol di-methyl ether 250 or polypropylene glycol 400 for separation of bovine serum albumin (BSA)

Zafarani-Moattar, Mohammed Taghi; Shekaari, Hemayat; Jafari, Parisa

doi:10.1016/j.molliq.2018.01.094

Cited by 34 publications

(22 citation statements)

References 56 publications

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“…[2] Notwithstanding, the hydrophilic choline-based ILs combined with PPG and PEG polymers have shown that higher temperatures triggers higher immiscibility regions. [24,25,26] Thus, the global conclusion achieved in this previous study [19] is consistent with our hypothesis. In the present research work, {K 3 PO 4 + ChGly + H 2 O} has resulted to be the limiting ABS.…”

Section: Biphasic and Three-phase Regions Dependences: Foundation Stosupporting

confidence: 92%

Setting the Foundations of Aqueous Three‐Phase Systems (A3PS) in the Quest for a Rational Design

et al. 2019

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This work presents the first in‐depth study of Aqueous Three‐Phase Systems (A3PS) with the main purpose of unveiling their behaviour, hence contributing to the development of this new field. Thus, a complete definition of a quaternary system was carried through by describing all the regions in detail to represent them later on in a regular‐tetrahedral diagram. The three aqueous faces of the tetrahedron demonstrated an undeviating influence in the segregation capacity. Furthermore, a method for comparing Aqueous Biphasis Systems (ABS) immiscibilities was set up in order to allow the evaluation and detection of the “limiting ABS” for the three‐phase region. Finally, all this information was compiled and utilised to obtain a new strategy for an A3PS rational design, which can be applied with ABS libraries or in an experimental approach. In this sense, this strategy represents an undoubted advance towards future studies and development of A3PS, as this sequential application of the constructed knowledge is assumed to save time and resources.

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Section: Biphasic and Three-phase Regions Dependences: Foundation Stosupporting

confidence: 92%

Setting the Foundations of Aqueous Three‐Phase Systems (A3PS) in the Quest for a Rational Design

et al. 2019

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“…By focusing on other proteins, Li et al [29] showed that ABSs comprising organic acid-derived cholinium-based IL and a polymer allow recovery yields between 86.4 and 99.9% of bovine serum albumin, trypsin, papain, and lysozyme. Zafarani-Moatar et al [31] used ABSs comprising amino acid-derived cholinium-based ILs and polymers to show that extraction efficiencies of bovine serum albumin as high as 85.91% are achievable. With ABSs formed by cholinium-based ILs bearing natural acids as the anions and a polymer, recovery yields of 20 to 100% were obtained for immunoglobulin G by Ramalho et al [28] and Mondal et al [30].…”

Section: Extraction Of Transferrinmentioning

confidence: 99%

“…Examples of bio-based ILs comprise those based on at least one of the following precursors: choline [13,14], glycine-betaine [15,16], guanidine [17,18], purine and pyrimidine nucleobases [19], carbohydrates [20,21], amino acids [14,22], organic acids [13,23], fatty acids [24,25], and terpenes [26,27]. Thus far, ILs bearing a cholinium cation combined with natural acid-derived anions are the most studied and used as proteinfriendly options in IL-based ABSs [28][29][30][31]. This work continues the pursuit for cheaper and bio-based ILs to form ABSs using formic and acetic acids as natural ILs' anion precursors.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Biphasic Systems Comprising Natural Organic Acid-Derived Ionic Liquids

et al. 2022

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Despite the progress achieved by aqueous biphasic systems (ABSs) comprising ionic liquids (ILs) in extracting valuable proteins, the quest for bio-based and protein-friendly ILs continues. To address this need, this work uses natural organic acids as precursors in the synthesis of four ILs, namely tetrabutylammonium formate ([N4444][HCOO]), tetrabutylammonium acetate ([N4444][CH3COO]), tetrabutylphosphonium formate ([P4444][HCOO]), and tetrabutylphosphonium acetate ([P4444][CH3COO]). It is shown that ABSs can be prepared using all four organic acid-derived ILs paired with the salts potassium phosphate dibasic (K2HPO4) and tripotassium citrate (C6H5K3O7). According to the ABSs phase diagrams, [P4444]-based ILs outperform their ammonium congeners in their ability to undergo liquid–liquid demixing in the presence of salts due to their lower hydrogen-bond acidity. However, deviations to the Hofmeister series were detected in the salts’ effect, which may be related to the high charge density of the studied IL anions. As a proof of concept for their extraction potential, these ABSs were evaluated in extracting human transferrin, allowing extraction efficiencies of 100% and recovery yields ranging between 86 and 100%. To further disclose the molecular-level mechanisms behind the extraction of human transferrin, molecular docking studies were performed. Overall, the salting-out exerted by the salt is the main mechanism responsible for the complete extraction of human transferrin toward the IL-rich phase, whereas the recovery yield and protein-friendly nature of these systems depend on specific “IL-transferrin” interactions.

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“…The frequently used ILs, consisting of imidazolium cation and bis (trifluoromethanesulfonyl)imide or hexafluorophosphate anions, are toxic and resistant to biodegradation . In this context, eco‐friendly ILs have been recently developed .…”

Section: Introductionmentioning

confidence: 99%

Comparison of three different dispersive liquid–liquid microextraction modes performed on their most usual configurations for the extraction of phenolic, neutral aromatic, and amino compounds from waters

Saraji

Ghambari

2018

J of Separation Science

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In this work we seek clues to select the appropriate dispersive liquid-liquid microextraction mode for extracting three categories of compounds. For this purpose, three common dispersive liquid-liquid microextraction modes were compared under optimized conditions. Traditional dispersive liquid-liquid microextraction, in situ ionic liquid dispersive liquid-liquid microextraction, and conventional ionic liquid dispersive liquid-liquid microextraction using chloroform, 1-butyl-3-methylimidazolium tetrafluoroborate, and 1-hexyl-3-methylimidazolium hexafluorophosphate as the extraction solvent, respectively, were considered in this work. Phenolic, neutral aromatic, and amino compounds (each category included six members) were studied as analytes. The analytes in the extracts were determined by high-performance liquid chromatography with UV detection. For the analytes with polar functionalities, the in situ ionic liquid dispersive liquid-liquid microextraction mode mostly led to better results. In contrast, for neutral hydrocarbons without polar functionalities, traditional dispersive liquid-liquid microextraction using chloroform produced better results. In this case, where dispersion forces were the dominant interactions in the extraction, the refractive index of solvent and analyte predicted the extraction performance better than the octanol/water partition coefficient. It was also revealed that none of the methods were successful in extracting hydrophilic analytes (compounds with the log octanol/water partition coefficient <2). The results of this study could be helpful in selecting a dispersive liquid-liquid microextraction mode for the extraction of various groups of compounds.

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Design of Novel Biocompatible and Green Aqueous two-Phase Systems containing Cholinium L-alaninate ionic liquid and polyethylene glycol di-methyl ether 250 or polypropylene glycol 400 for separation of bovine serum albumin (BSA)

Cited by 34 publications

References 56 publications

Setting the Foundations of Aqueous Three‐Phase Systems (A3PS) in the Quest for a Rational Design

Setting the Foundations of Aqueous Three‐Phase Systems (A3PS) in the Quest for a Rational Design

Aqueous Biphasic Systems Comprising Natural Organic Acid-Derived Ionic Liquids

Comparison of three different dispersive liquid–liquid microextraction modes performed on their most usual configurations for the extraction of phenolic, neutral aromatic, and amino compounds from waters

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