Design of novel functionalized collagen-chitosan-MBG scaffolds for enhancing osteoblast differentiation in BMSCs

Abstract

Collagen and chitosan are two different kinds of natural biodegradable polymers commonly used in the regeneration of bone defects. Mesoporous bioactive glass (MBG) is a type of favorable bone filler which can effectively constitute an enlarged microenvironment to facilitate an exchange of important factors between the cells and scaffolds. Here we prepared a collagen-chitosan-MBG (C-C-MBG) scaffold which displayed significantly increased proliferation, differentiation and mineralization in bone mesenchymal stem cells (BMSCs). Additionally, we found that the scaffold can stimulate extra-cellular signal regulated kinase 1/2 (Erk1/2) activated Runx2 pathway, which is the predominant signaling pathway involved in osteoblast differentiation. Consistently, we observed that the scaffold can markedly enhance the expression of Type I collagen, Osteopontin (Opn), and Runx2, which are important osteoblastic marker genes implicated in the process of osteoblast differentiation. Therefore, we conclude that the composite scaffold can significantly promote the differentiation of BMSCs into osteoblasts by activating Erk1/2-Runx2 pathway. Our finding thereby implies that the C-C-MBG scaffold can possibly act as a potential biomaterial in the bone regeneration.

Ce-doped Nanobioactive Glass/ Collagen/ Chitosan Composite Scaffolds: Biocompatibility with Normal Rabbit’s Osteoblast Cells and Anticancer Activity Test

“…Despite the advantages of collagen and chitosan scaffolds in the tissue engineering the main drawback is their biological inertness aspect. Accordingly, bioactive fillers (such as, bioactive glass) have been suggested to be incorporated in this kind of polymers to improve their bioactivity (Kaczmarek et al, 2020;Gao et al, 2021;Deen et al, 2022). Moreover, the scaffold can be functionalized with by doping of bioactive glass filler with therapeutic ions such as, silver, copper and cerium, in these fillers.…”

Composite Matrixes for Use in Traumatology and Regenerative Medicine

“…Также они могут стимулировать экспрессию транскрипционного фактора Runx2, активируемого киназой Erk1/2. Этот сигнальный путь является одним из главных путей в дифференцировке остеобластов [19]. Кроме того, было установлено, что композитные скаффолды из хитозана-коллагена с добавлением фиброина шелка являются биологически совместимыми с остеобластоподобными клетками MG-63 [20].…”