Design of Peptide Ligand for Lactoferrin and Study of Its Binding Specificity

Zimina, Tatiana M.; Sitkov, Nikita O.; Карасев, В. Е.; Skorik, Yury А.; Kolobov, A. A.; Буненков, Н. С.; Luchinin, V. V.

doi:10.3390/chemosensors11030162

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…The electrophoretic homogeneity of synthetic peptide containing 21 AA residues proposed as lactoferrin binding ligand by in silico modeling was confirmed by CE analysis in BFS capillary (75 µm id, 60/50 cm total/effective length) thermostated at 30°C using 10 mM Tris/HCl buffer, pH 7.2, as BGE, 20 kV as separation voltage, and 200 nm as detection wavelength [229]. The CE analysis of a mixture of this peptide with lactoferrin proved formation of peptide–lactoferrin complex.…”

Section: Discussionmentioning

confidence: 98%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Discussionmentioning

confidence: 98%

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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“…Peptides have a number of advantages over more widely used antibodies and aptamers, such as low cost, ease of synthesis and modification, high stability and regenerability, low immunogenicity and toxicity, and the ability to be designed as needed [228]. However, peptides also have a number of disadvantages, such as low affinity and specificity compared to antibodies, low catalytic activity compared to enzymes, and the potential for denaturation, aggregation, and degradation under certain conditions.…”

Section: Peptide "Aptamers"mentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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“…electrophoresis techniques to study its binding specificity [55]. Shahzadi et al explored the feasibility of leveraging ultra-thin 2D materials, renowned for their high affinity for proteins and DNA, for biosensing applications [56].…”

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confidence: 99%