Design of poly(N‐acryloylglycine) materials for incorporation of microorganisms

Abstract

1097-4628International audienceTo incorporate microorganisms and to preserve their integrity, new matrices of poly(N-acryloylglycine) have been designed under appropriate conditions. To understand the interactions between the microorganisms and the organic part of the matrices, different conetworks of poly(N-acryloylglycine) have been synthesized and characterized. Copolymerization with two crosslinkers was performed with different compositions. The thermal and swelling properties of conetworks are specifically controlled and compared. These investigations show that the swelling ratio of these materials is compatible with the incorporation of biomolecules in these matrices. They successfully permit Pseudomonasspecies 1625 bacteria incorporation. The biological activity of bacteria is also preserved, allowing the use of these materials for innovative biological applications. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 835-841, 201

Poly(N-acryloylglycine-acrylamide) Hydrogel Mimics the Cellular Microenvironment and Promotes Neurite Growth with Protection from Oxidative Stress

“…A second endothermic transition was observed at 210 °C due to the melting of the block copolymer hydrogel. Exothermic heat flow was observed from 300 °C and above, due to the degradation (weight loss) of the polymer, which matched well with the TGA (Figure S7a) and was obtained due to the degradation of polyamide ketone and nitrile derivatives . The acrylamide was cross-linked with DVB, and due to heating, the evolution of NH 3 gas was started at 210 °C, which can be correlated with the TGA results of p­(NAG- b -A) .…”

RP-UHPLC-MS Chemical Profiling, Biological and In Silico Docking Studies to Unravel the Therapeutic Potential of Heliotropium crispum Desf. as a Novel Source of Neuroprotective Bioactive Compounds

“…A total of 13 known nitrogen-containing secondary metabolites were tentatively identified through negative ionization mode, including mostly cyclic and acyclic peptides derivatives; 5-acetylamino-6-formylamino-3-methyluracil ( 7 ), 1-methylxanthine ( 8 ), and hypoxanthine ( 9 ) of the xanthine family compounds [ 17 ]. N -Acryloylglycine ( 10 ) and orysastrobin ( 11 ) are strobilurins derivatives [ 18 ], which are widely used as a fungicide [ 19 ]. Other identified compounds, including mecarbinzid ( 12 ), the oxidized compound oplophorus luciferin ( 13 ), and biotin-X-NHS ( 14 ), are important secondary metabolites.…”

Multiresponsive Poly(N-Acryloyl glycine)-Based Nanocomposite and Its Drug Release Characteristics

“…Magnetite nanoparticle (MNP) has attracted great attention in recent years in biomedical and biotechnological applications [1][2][3][4][5] owing to its magnetically guidable properties [2], high surface area-to-volume ratio [4], high saturation magnetization [6,7], low toxicity, and high biocompatibility [8]. These intriguing properties make MNP as an ideal candidate for use in various biomedical fields such as drug delivery [5], diagnostics, therapeutics [2,9,10], and magnetic separation [11][12][13].…”

“…When individual unique properties of both MNP and polymer matrix were combined, multifunctional nanocomposite serving as a platform for further conjugation with desirable bioentities can be obtained [16,17]. Thus, this hybrid nanocomposite has been particularly used in the biological field such as drug delivery system [18,19], controlled release [16,20,21], and magnetic separation [11][12][13]. Previous works have reported the synthesis of MNP-polymer nanocomposite having both good magnetic separation ability and good water dispersibility for drug controlled release [4,16] and for conjugation with bioentities [17,22].…”

“…Previous works have presented the use of MNP coated with pHand thermoresponsive polymers as a handle for triggered mechanisms for drug controlled release [26]. Among the pH-and thermo-responsive polymers, poly(N-acryloyl glycine) (PNAG) is of particular interest in this research because it can be facilely synthesized via a free radical polymerization of N-acryloyl glycine monomer in aqueous solutions [12,28]. H-bonding network of carboxyl groups (-COOH) and amide groups (-CONH-) in PNAG chains with water molecules plays a crucial role in its pH-and temperature-responsive properties [29].…”