Design of Polymeric Gene Carriers for Effective Intracellular Delivery

Lai, Wing‐Fu; Wong, Wing Tak

doi:10.1016/j.tibtech.2018.02.006

Cited by 119 publications

(94 citation statements)

References 81 publications

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“…Owing to their positive surface charge, the NCs were shown to condense nucleic acids during the gel retardation assay . Simply possessing the capacity of condensing nucleic acids, however, is not sufficient for gene delivery, which involves other processes such as cellular internalization and endo‐lysosomal escape . Detailed evaluation of the in vitro and in vivo transfection efficiency of the NCs is required before the application potential of the NCs for gene therapy can be more conclusively determined.…”

Section: Synthesis Of Cu Ncs As Theranostic Agentsmentioning

confidence: 99%

“… Diagnosis using Cu NCs is performed largely based on the detection of abnormal molecular events in the pathological site. In cancer cells, abnormal molecular events are usually manifested in terms of alternations in protein expression, changes in the level of reactive oxygen/nitrogen species, and alternations in intracellular pH . Cu NCs can possibly be developed as probes for these molecular events because some of them exhibit an aggregation‐induced emission (AIE) effect in liquid media .…”

Section: Working Principles Of Cu Ncs As Theranostic Agentsmentioning

confidence: 99%

“…This has been suggested by an earlier study, [93] which observed no cytotoxicity in A549 cells after 24 h exposure of the cells to 30 nm diameter CuS clusters at a concentration of 800 × 10 −6 m. Upon a reduction in the size of the clusters to around 5 nm, exposure of the cells to a concentration of 50 × 10 −6 m was found to cause the cell viability to drop to 15%. [93] This suggests that a reduction in size may cause an increase in the efficiency of cellular internalization of the NCs, [52] thereby increasing the cellular content of the clusters and hence the effect of the clusters on the physiology of the cells. Despite the close relationship between the NC size and the physiological activity, ultrasmall size is an intrinsic characteristic of Cu NCs.…”

Section: Size Effectsmentioning

confidence: 99%

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Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

2020

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Theranostics refers to the incorporation of therapeutic and diagnostic functions into one material system. An important class of nanomaterials exploited for theranostics is metal nanoclusters (NCs). In contrast to gold and silver NCs, copper is an essential trace element for humans. It can be more easily removed from the body. This, along with the low cost of copper that offers potential large‐scale nanotechnology applications, means that copper NCs have attracted great interest in recent years. The latest advances in the design, synthesis, surface engineering, and applications of copper NCs in disease diagnosis, monitoring, and treatment are reviewed. Strategies to control and enhance the emission of copper NCs are considered. With this synopsis of the up‐to‐date development of copper NCs as theranostic agents, it is hoped that insights and directions for translating current advances from the laboratory to the clinic can be further advanced and accelerated.

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Section: Synthesis Of Cu Ncs As Theranostic Agentsmentioning

confidence: 99%

Section: Working Principles Of Cu Ncs As Theranostic Agentsmentioning

confidence: 99%

Section: Size Effectsmentioning

confidence: 99%

See 1 more Smart Citation

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

2020

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“…Inspired by virus strategies that use multiple attachment factors and receptors for targeting host cells in parallel or in succession, researchers have exploited various ligands conjugated to delivery systems aiming to facilitate tumor cell targeting and penetration. To date, various cell specific ligands have been employed, such as lactose, anti‐CD3 antibody, OV‐TL16, PSMA‐specific monoclonal antibody J591, HER‐2 antibody, mannose, GRP‐78‐targeting peptide, transferrin, RGD peptide and folic acid . Sun et al .…”

Section: Specificity and Infection Properties Mimic Designs For Targementioning

confidence: 99%

“…65 employed, such as lactose, anti-CD3 antibody, OV-TL16, PSMAspecific monoclonal antibody J591, HER-2 antibody, mannose, GRP-78-targeting peptide, transferrin, RGD peptide and folic acid. 75 Sun et al 76 developed a targeted gene delivery system by conjugating βcyclodextrin modified polyethylenimine (PEI-CD) and adamantyl peptide (AdGRGDS) based on host-guest interaction. In vitro and in vivo assays highlighted the importance of AdGRGDS targeting specificity to tumor cells with integrin α v β 3 overexpression and demonstrated that the PEI-CD/AdGRGDS gene delivery system had great potential for targeted tumor therapy.…”

Section: Specificity and Infection Properties Mimic Designs For Tarmentioning

confidence: 99%

Virus‐inspired and mimetic designs in non‐viral gene delivery

Luo

Liang

Jin

et al. 2019

The Journal of Gene Medicine

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Virus‐inspired mimics for nucleic acid transportation have attracted much attention in the past decade, especially the derivative microenvironment stimuli‐responsive designs. In the present mini‐review, the smart designs of gene carriers that overcome biological barriers and realize an efficient delivery are categorized with respect to the different “triggers” provided by tumor cells, including pH, redox potentials, ATP, enzymes and reactive oxygen species. Some dual/multi‐responsive gene vectors have also been introduced that show a more precise and efficient delivery in the complicated environment of human body. In addition, inspired by the special recognition mechanisms and components of viruses, improvements in the design of carriers relating to targeting/penetration properties, as well as chemical component evolution, are also addressed.

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Nanotechnology Responses to COVID‐19

Ruiz-Hitzky

Darder

Wicklein

et al. 2020

Adv Healthcare Materials

144

134

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Dedicated to Dr. André Van Meerbeeck and to all the direct and indirect victims of the COVID-19 pandemic Researchers, engineers, and medical doctors are made aware of the severity of the COVID-19 infection and act quickly against the coronavirus SARS-CoV-2 using a large variety of tools. In this review, a panoply of nanoscience and nanotechnology approaches show how these disciplines can help the medical, technical, and scientific communities to fight the pandemic, highlighting the development of nanomaterials for detection, sanitation, therapies, and vaccines. SARS-CoV-2, which can be regarded as a functional core-shell nanoparticle (NP), can interact with diverse materials in its vicinity and remains attached for variable times while preserving its bioactivity. These studies are critical for the appropriate use of controlled disinfection systems. Other nanotechnological approaches are also decisive for the development of improved novel testing and diagnosis kits of coronavirus that are urgently required. Therapeutics are based on nanotechnology strategies as well and focus on antiviral drug design and on new nanoarchitectured vaccines. A brief overview on patented work is presented that emphasizes nanotechnology applied to coronaviruses. Finally, some comments are made on patents of the initial technological responses to COVID-19 that have already been put in practice.

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Design of Polymeric Gene Carriers for Effective Intracellular Delivery

Cited by 119 publications

References 81 publications

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

Development of Copper Nanoclusters for In Vitro and In Vivo Theranostic Applications

Virus‐inspired and mimetic designs in non‐viral gene delivery

Nanotechnology Responses to COVID‐19

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