Polyphase filter banks (PFBs) are the most preferred multirate structures for subband coding in Digital Signal Processing (DSP) and communication. For PFB design, there are many important design parameters such as filter length and frequency selectivity. Also, to realize the desired frequency response in designs, stopband and passband attenuation are of considerable importance. In PFB design, researchers and practitioners frequently use iterative and meta-heuristic optimization methods. Heuristic techniques have a significant problem-solving ability in continuous and discrete solution space. Therefore, they give better results than other suggested methods, and their performance depends on the control parameters. In this study, Artificial Bee Colony (ABC) algorithm was employed for suggested design problem of PFB. In the first stage, the control parameters of the ABC algorithm were examined to improve the performance of the proposed PFB problem. In the second stage, the analysis was carried out by changing filter lengths (8-256) and filter band frequencies (0.3-0.7/0.4-0.6). All results obtained were also compared with the Particle Swarm Optimization algorithm (PSO) and the Genetic algorithm (GA). Finally, a DSP application of PFB was carried out according to best results achieved by the ABC algorithm for filter lengths and frequencies.