Design of polysaccharidic Aloe vera gel incorporated PVA/tetracycline electrospun cell culture scaffolds for biomedical applications

Abstract

In this study, hybrid nanofibrous 3D scaffolds containing Aloe vera (AV), polyvinyl alcohol (PVA) and tetracycline hydrochloride (TCH) are fabricated by electrospinning for cell culture applications. The role of polysaccharides present in AV gel is found to enhance the biocompatibility of the nanofibrous scaffolds. Different combinations of the polymers were selected to produce homogenous nanofibers with favorable mean fiber diameter and tensile strength. The surface morphology of the products was studied by SEM and it is found that the mean fiber diameter is decreased to about 188 nm upon addition of the AV component. The electrospun scaffolds were investigated by FT-IR spectroscopy to reveal the chemical structure of the samples and their crystallinity was studied by XRD. The hydrophilicity of the scaffolds was tested by optical contact angle measurements and their mechanical strength was examined by tensile strength tests. It is found that PVA is the main component contributing the mechanical stability of the scaffold structure. The fabricated scaffolds presented a more pronounced inhibitory effect against Gram-positive bacterial strains of S. aureus and B. cereus. Cell culture experiments using fibroblast L929 murine cells reveals that the AV/PVA/TCH scaffolds are promising for cell growth and the cells are capable of achieving a proper cell adhesion and proliferation. The cell viability experiment by MTT assay exhibits the contributing role of AV gel to L929 cell viability on the AV/PVA/TCH scaffolds.

Preparation and Characterization of Chitosan Nanofiber: Kinetic Studies and Enhancement of Insulin Delivery System

“…The next SEM image is of the main layer (batchS1), which consists of insulin, CS, and PEO. CS is a supporting material, to which PEO was added to improve the fiber properties [ 29 , 30 ], as shown in the image in Figure 6 . The micrographs show nanofibers, and their size distribution curves show that 4% have diameters in the range of 0–50 nm and 52% have diameters in the range of 50–100 nm.…”

Effect of tetracycline hydrochloride application on dental pulp stem cell metabolism–booster or obstacle for tissue engineering?

“…To address this problem, several researchers in recent years have attempted to combine TCH with various organic polymers, especially degradable ones, to simultaneously function as a cellular scaffold and a drug delivery system. Allafchian et al combined TCH with polysaccharide aloe vera gel and polyvinyl alcohol to form a cellular scaffold and found good antibacterial activity against Staphylococcus aureus and Bacillus cereus without affecting cell adhesion and proliferation ( Allafchian et al, 2022 ). Ferreira et al used a cellular scaffold combining TCH, metronidazole, and electrostatic spinning for the treatment of rats in a periodontitis model and found increased new bone formation, decreased bone loss, and reduced inflammatory response ( Ferreira et al, 2021 ).…”

Polymeric Nanofibers for Drug Delivery Applications: A Recent Review

“…Also, different levels of bioactive components from the Chinese herbal medicine dandelion were loaded into the electrospinning solution to create composite nanofiber mats to investigate the wound dressing properties of composite nanofiber materials and their ability to treat diabetic wounds. In addition, Allafchian et al [ 54 ] prepared 3D nanofiber scaffolds containing aloe vera, polyvinyl alcohol, and tetracycline hydrochloride by electrostatic spinning method for cell culture applications. However, when employing electrospinning technology to get uniform fibers, it must be equipped with a strong electric field, the production efficiency is low, and it is difficult to combine into a large-scale 3D network, all of which limit the technology’s popularity and application [ 55 – 57 ].…”