Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

Abstract

Hybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se , showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications.

Stabilization of b-Glucuronidase by Immobilization in Magnetic-Silica Hybrid Supports

“…The difference was particularly significant when 100 nm MNPs (BioSi@β-Glc _Amine) were used instead of 200 nm MNPs (BioSi@β-Glc _PAA and BioSi@β-Glc_Doxi). Regardless of the impact that the size of the MNPs could have, the results correlate with those previously demonstrated by Correa et al [13] where the MNPs were found to have a profound effect on the silica matrix formation irrespective of the size of the aminated mediator of the Si synthesis. The values for polydispersity index of all the preparations vary around 0.3, indicating a homogeneous population of particles with a low probability of agglomeration.…”

“…The properties of immobilized biocatalysts on hybrid materials are partially determined by the individual effect of each material on the enzyme upon immobilization [13]. Therefore, as a first step in the integration of β-GIc in a material containing silica (Si) and MNPs, we studied the entrapment of the enzyme solely in biomimetic Si.…”

“…It was not possible to establish a different stabilization factor for the Si alone and the combined Si-MNPs materials as both preparations showed outstanding stability in the conditions tested compared to the soluble enzyme. However, MNPs provides the obvious advantage of ease of separation after desired applications and could also serve as a mean to increase the enzymatic activity via magnetic hyperthermia [13].…”

“…A recent trend in the field of enzyme immobilization is the use of combined or hybrid materials [12,13]. In this approach, different materials are involved with a specific aim in aiding the catalysis, the stability of the biocatalysts, or providing an added advantageous property.…”

Reflections on the 2nd International Congress on NanoBioEngineering 2020

“…Next, León-Buitimea et al talked about the role of nanobioengineering in the development of smart therapeutics with programmed dynamic responses to target, release, and clear therapeutics in an efficient manner in the treatment of infectious diseases caused by virus and bacteria (León-Buitimea et al, 2020 ). Finally, Correa et al described the advances in her laboratory toward the design of hybrid composite nanoparticles that integrate enzymes to incorporate catalytic and superparamagnetic behaviors that would aid in designing drug delivery systems (Correa et al, 2019 ). The session was complemented with participants who exposed their research work on developing new drug delivery systems based on nanobioengineering to tackle different diseases, including tuberculosis, viral infections, including COVID-19, and cancer.…”