Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture

Akimoto, Aya Mizutani; Hasuike, Erika; Tada, Hiroaki; Nagase, Kenichi; Okano, Teruo; Kanazawa, Hideko; Yoshida, Ryo

doi:10.2116/analsci.32.1203

Cited by 16 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…These systems have found utility in neural repair, as they support localized treatment and minimize postsurgical complications, demonstrating versatility for translation to the clinic. , Injectable materials permit the formation of a hydrogel in situ via the minimally invasive delivery of a hydrogel precursor to the desired location. Once injected, the hydrogel can be formed using a variety of physical or covalent cross-linking methods, including environmental stimuli such as temperature, pH and salt concentration. ,− Both natural and synthetic polymers can be designed to be injectable, such as chitosan-based thermoresponsive hydrogels or injectable PEG polymers. , The combination of hydrogel precursor and method of polymerization will determine the final molecular arrangement, allowing for finely controlled macromolecular conformations. , …”

Section: Introductionmentioning

confidence: 99%

“…20,42−45 Both natural and synthetic polymers can be designed to be injectable, such as chitosan-based thermoresponsive hydrogels or injectable PEG polymers. 46,47 The combination of hydrogel precursor and method of polymerization will determine the final molecular arrangement, allowing for finely controlled macromolecular conformations. 48,49 The class of injectable materials termed self-assembling, offer a thermodynamic advantage by exploiting spontaneous physical interactions of the molecules in the environment, forming stable network microstructures.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Self-Assembling Hydrogel Structures for Neural Tissue Repair

Peressotti

Koehl

Goding

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Hydrogel materials have been employed as biological scaffolds for tissue regeneration across a wide range of applications. Their versatility and biomimetic properties make them an optimal choice for treating the complex and delicate milieu of neural tissue damage. Aside from finely tailored hydrogel properties, which aim to mimic healthy physiological tissue, a minimally invasive delivery method is essential to prevent off-target and surgery-related complications. The specific class of injectable hydrogels termed self-assembling peptides (SAPs), provide an ideal combination of in situ polymerization combined with versatility for biofunctionlization, tunable physicochemical properties, and high cytocompatibility. This review identifies design criteria for neural scaffolds based upon key cellular interactions with the neural extracellular matrix (ECM), with emphasis on aspects that are reproducible in a biomaterial environment. Examples of the most recent SAPs and modification methods are presented, with a focus on biological, mechanical, and topographical cues. Furthermore, SAP electrical properties and methods to provide appropriate electrical and electrochemical cues are widely discussed, in light of the endogenous electrical activity of neural tissue as well as the clinical effectiveness of stimulation treatments. Recent applications of SAP materials in neural repair and electrical stimulation therapies are highlighted, identifying research gaps in the field of hydrogels for neural regeneration.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Self-Assembling Hydrogel Structures for Neural Tissue Repair

Peressotti

Koehl

Goding

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

“…The colorless and transparent PNIPAAm gel was obtained by mixing PNIPAAm derivatives (main chain polymer, providing amino moieties), N -hydroxysuccinimide-terminated octa-branched polyethylene glycol (NHS-octa-PEG) (crosslinker polymer), and RGDS peptides, in the so-called post-crosslinking method [ 14 ]. This method is advantageous for cell culture applications because the gelation process does not contain harmful compounds such as monomers, and the procedure makes it easy to prepare gels inside cell culture dishes or wells of micro-plates.…”

Section: Resultsmentioning

confidence: 99%

“…PNIPAAm hydrogels and PDMAAm hydrogels as a control were synthesized by the post-crosslinking method slightly modified from our previous study [ 14 ]. First, NIPAAm (or DMAAm) and NAPMAm were copolymerized by free radical polymerization (molar ratio of NIPAAm or DMAAm:NAPMAm = 98:2).…”

Section: Methodsmentioning

confidence: 99%

Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation

Akimoto

Niitsu

Nagase

et al. 2018

IJMS

Self Cite

View full text Add to dashboard Cite

We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa-arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow-derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post-crosslinking gelation method was revealed to be cytocompatible and showed temperature-dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

show abstract

Evaporative Cooling Hydrogel Packaging for Storing Biologics Outside of the Cold Chain

Ferber

Behrens

McHugh

et al. 2018

Adv Healthcare Materials

View full text Add to dashboard Cite

Stabilizing thermolabile pharmaceuticals outside of the cold chain has the potential to alleviate some of the logistical and monetary burden of providing health care access in the developing world. Evaporative cooling hydrogel packaging is designed to extend the storage stability of existing pharmaceutical products without the need for reformulation. Hydrogels with high water content and reversible hydrophilicity offer a promising platform for reducing storage temperatures without refrigeration. As a model, poly(N-isopropylacrylamide) is selected as a basis for creating a potentially low cost and easy-to-fabricate hydrogels.

show abstract

Design of Tetra-arm PEG-crosslinked Thermoresponsive Hydrogel for 3D Cell Culture

Cited by 16 publications

References 20 publications

Self-Assembling Hydrogel Structures for Neural Tissue Repair

Self-Assembling Hydrogel Structures for Neural Tissue Repair

Mesenchylmal Stem Cell Culture on Poly(N-isopropylacrylamide) Hydrogel with Repeated Thermo-Stimulation

Evaporative Cooling Hydrogel Packaging for Storing Biologics Outside of the Cold Chain

Contact Info

Product

Resources

About