Aimed at the problems of low reproductive coefficient and long seeding period for Mandevilla sanderi 'Sunparasol', the rapid propagation system was studied in this article. Research results showed as follows: The disinfectant and disinfection time for young stem segments of M.sanderi is 0.1% HgCl2 for 3 min; When the culture medium is MS+6-BA 4 mg/L+NAA 0.1 mg/L, the germination rate is as high as 93.33%; In the subculture stage, the number of multiplication and growth is strong in the medium of MS+6-BA 2 mg/L+NAA 0.1 mg/L, and the multiplication coefficient is 4.31; Sucrose concentration of 40 g/L can promote the growth of fine tissue culture seedlings; the optimum growth condition of test tube seedlings is the best when 1~2 mg/L of CCC is added; The suitable rooting medium is 1/2MS+NAA 0.1 mg/L, the rooting rate is 83.33%, and the average rooting number is 4.3. The above-mentioned establishment of tissue culture rapid propagation system has important guiding significance for the large-scale production of M.sanderi.