This paper presents a prototype of a disinfection system for public transport specifically aiming to disinfect surfaces contaminated with the SARS-CoV-2 virus on buses using 222 nm wavelength far-ultraviolet light (far-UVC). Our study involved testing the developed technical system installed in a 12 m long M3 category urban bus, an investigation of optimal far-UVC light angles, and the determination of disinfection parameters for bus seat disinfection. The study identified the ideal positioning of a light source for effective disinfection and analyzed three disinfection scenarios, considering zone coverage, disinfection time, and energy demand. A subsystem employing real-time occupancy monitoring enhances the disinfection process in crowded areas of buses. An energy efficiency assessment model is proposed for optimizing energy consumption. Furthermore, the energy consumption analyses in different disinfection scenarios provide valuable insights for optimizing energy usage in public transport disinfection.