Design of vitrification solutions for the cryopreservation of embryos

Ali, Jawad K.; Shelton, J. N.

doi:10.1530/jrf.0.0990471

Cited by 168 publications

(68 citation statements)

References 13 publications

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“…Until now, vitrification has been adopted to cryopreserve embryos [3,4], oocytes [5][6][7][8][9] and ovarian tissue [10][11][12][13]. Kuwayama reported that vitrification, compared with slow cooling, resulted in high survival rates for all stages of embryo development [14].…”

Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

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Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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“…17 Our understanding of vitrification solutions and their potential toxicity to embryos was enhanced by the efforts of a few insightful pioneers in the field of reproductive cryobiology. [18][19][20][21][22] Their efforts led to the combined use of mixed permeating CPAs (eg, ethylene glycol [EG], dimethyl sulfoxide [DMSO] and glycerol), as well as non-permeating solutes (eg, sucrose and ficoll) that factored into reducing toxicity potential of individual vitrification solutions.…”

Section: Insights Into the Pioneering Past Of Vitrificationmentioning

confidence: 99%

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

Schiewe¹,

Anderson²

2017

BSAM

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After more than 2 decades of development in mammalian models and the clinical focus of a few pioneering laboratories, vitrification of human oocytes and embryos has transformed today's assisted reproductive technology (ART) industry. Our ability to cryopreserve gametes and embryos without fear of the damaging effects of ice formation has instilled great confidence in post-warming specimen viability. In turn, clinical treatment options are progressively eliminating fresh embryo transfer (ET; ie, freeze-all cycles), integrating preimplantation genetic screening and contemplating the use of cryopreserved oocytes as a viable resource. Vitrification's impact on clinical treatments is akin to the advent of sperm injection technology in the 1990s on male factor infertility. An appreciable and quantifiable difference was made, with procedural efficacy and global reliability essentially being guaranteed. Yet, there are challenges in the current trends and perspectives in how this technology is and will be optimized in the future. User variation in vitrification products and procedures warrants stricter adherence to quality control measures to enhance specimen biosafety and patient satisfaction, while reducing potential liability concerns. Furthermore, future progress in our understanding of the chemical and cryophysical processes of vitrification will insure the effective cryostorage of reproductive tissues and gametes at a level attained for embryo cryopreservation today.

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“…Furthermore, our understanding of vitrification solutions and their potential toxicity were enhanced by Drs. Jill Shaw [49,50], Jaffar Ali [51], Miyake Kasai [52] and Tetsunori Mukaida [53] and their co-workers. By the mid-2000's, it was the commercial industry, developing new devices and solutions that propelled vitrification use into IVF labs, for better or worse.…”

Section: Vitrificationmentioning

confidence: 99%

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

Schiewe¹

2016

JFIV Reprod Med Genet

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The concept of assisted reproductive technologies was most notably derived from the late 19th Century experiments of Sir Walter Heape who successfully transplanted rabbit embryos. Interestingly, it was not until the late 1940's and 1950's that renewed interests in rabbit embryo transfer and cryobiology occurred. The history behind developing effective procedures can be fascinating, though few could be more accidental than Dr. Chris Polge's discovery of glycerol (1948), from a mislabeled bottle of sugar solutions, being an effective cryoprotective agent for sperm freezing. The purpose of this review paper is to discuss four key scientific breakthrough technologies occurring between 1985 and 1995, which ultimately shaped the future of today's human in vitro fertilization (IVF) industry. More importantly, this paper highlights the foundation of underlying related discoveries and some unique stories involving their development and publication. In the end, this paper emphasizes the value of understanding scientific discovery timetables and the eventual re-discovery in the hands and minds of creative, determined and dedicated scientists, as history tends to repeat itself before its useful application is realized.

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Design of vitrification solutions for the cryopreservation of embryos

Cited by 168 publications

References 13 publications

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Vitrification: the pioneering past to current trends and perspectives of cryopreserving human embryos, gametes and reproductive tissue

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

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