Design Principles of the Rotary Type 9 Secretion System

Trivedi, Abhishek; Gosai, Jitendrapuri; Nakane, Daisuke; Shrivastava, Abhishek

doi:10.3389/fmicb.2022.845563

Cited by 20 publications

(25 citation statements)

References 103 publications

(167 reference statements)

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“…Highly active genes within this transcriptome belong to T9SS mechanism and gliding motility ( Supplementary Table S4 ). Overall, 18 genes ( gldA , gldB , gldD , gldF , gldG , gldH , gldI , gldJ , gldK , gldL , gldM , gldN , sprA , sprE , sprF , sprT , porU and porV ), required for gliding motility and protein secretion, and/or involved in T9SS ( Hunnicutt and Mcbride, 2000 ; McBride and Braun, 2004 ; Braun et al, 2005 ; Lauber et al, 2018 ; McBride, 2019 ; Hennell James et al, 2021 ; Trivedi et al 2022 ; Veith et al, 2022 ), were identified among DEGs ( Supplementary Table S2 ).…”

Section: Resultsmentioning

confidence: 99%

Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091

et al. 2022

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Based on previous research, related to detailed insight into mutualistic collaboration of microalga and its microbiome, we established an artificial plant-bacteria system of the microalga Micrasterias radians MZCH 672 and the bacterial isolate Dyadobacter sp. HH091. The bacteria, affiliated with the phylum Bacteroidota, strongly stimulated growth of the microalga when it was added to axenic algal cultures. For further advances, we studied the isolate HH091 and its interaction with the microalga M. radians using transcriptome and extensive genome analyses. The genome of HH091 contains predicted polysaccharide utilizing gene clusters co-working with the type IX secretion system (T9SS) and conceivably involved in the algae-bacteria liaison. Here, we focus on characterizing the mechanism of T9SS, implementing the attachment and invasion of microalga by Dyadobacter sp. HH091. Omics analysis exposed T9SS genes: gldK, gldL, gldM, gldN, sprA, sprE, sprF, sprT, porU and porV. Besides, gld genes not considered as the T9SS components but required for gliding motility and protein secretion (gldA, gldB, gldD, gldF, gldG, gldH, gldI, gldJ), were also identified at this analysis. A first model of T9SS apparatus of Dyadobacter was proposed in a course of this research. Using the combination of fluorescence labeling of Dyadobacter sp. HH091, we examined the bacterial colonisation and penetration into the cell wall of the algal host M. radians MZCH 672.

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Section: Resultsmentioning

confidence: 99%

Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091

et al. 2022

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“…In vivo orientation measurement through single-molecule polarization microscopy (Shroder et al, 2016) of fluorescently labeled proteins is another possible approach to circumvent the difficulty of labeling periplasmic proteins with microparticles. The Bacteroidetes gliding system is reviewed elsewhere in this collection (Trivedi et al, 2022).…”

Section: Gldlm Bacteroidetes Gliding Systemmentioning

confidence: 99%

A new class of biological ion-driven rotary molecular motors with 5:2 symmetry

et al. 2022

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Several new structures of three types of protein complexes, obtained by cryo-electron microscopy (cryo-EM) and published between 2019 and 2021, identify a new family of natural molecular wheels, the “5:2 rotary motors.” These span the cytoplasmic membranes of bacteria, and their rotation is driven by ion flow into the cell. They consist of a pentameric wheel encircling a dimeric axle within the cytoplasmic membrane of both Gram-positive and gram-negative bacteria. The axles extend into the periplasm, and the wheels extend into the cytoplasm. Rotation of these wheels has never been observed directly; it is inferred from the symmetry of the complexes and from the roles they play within the larger systems that they are known to power. In particular, the new structure of the stator complex of the Bacterial Flagellar Motor, MotA5B2, is consistent with a “wheels within wheels” model of the motor. Other 5:2 rotary motors are believed to share the core rotary function and mechanism, driven by ion-motive force at the cytoplasmic membrane. Their structures diverge in their periplasmic and cytoplasmic parts, reflecting the variety of roles that they perform. This review focuses on the structures of 5:2 rotary motors and their proposed mechanisms and functions. We also discuss molecular rotation in general and its relation to the rotational symmetry of molecular complexes.

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“…One possibility is that the rotating motor pushes on a tread carrying SprB filaments and propels it along the helical GldJ track 4 , 24 . Alternatively, the SprB filaments could be anchored on the tracks, and the tracks could be propelled by the motor 24 , 58 .…”

Section: Discussionmentioning

confidence: 99%

Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

et al. 2023

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Many bacteria belonging to the phylum Bacteroidetes move on solid surfaces, called gliding motility. In our previous study with the Bacteroidetes gliding bacterium Flavobacterium johnsoniae, we proposed a helical loop track model, where adhesive SprB filaments are propelled along a helical loop on the cell surface. In this study, we observed the gliding cell rotating counterclockwise about its axis when viewed from the rear to the advancing direction of the cell and revealed that one labeled SprB focus sometimes overtook and passed another SprB focus that was moving in the same direction. Several electron microscopic analyses revealed the presence of a possible multi-rail structure underneath the outer membrane, which was associated with SprB filaments and contained GldJ protein. These results provide insights into the mechanism of Bacteroidetes gliding motility, in which the SprB filaments are propelled along tracks that may form a multi-rail system underneath the outer membrane. The insights may give clues as to how the SprB filaments get their driving force.

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Design Principles of the Rotary Type 9 Secretion System

Cited by 20 publications

References 103 publications

Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091

Interplay between the microalgae Micrasterias radians and its symbiont Dyadobacter sp. HH091

A new class of biological ion-driven rotary molecular motors with 5:2 symmetry

Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

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