Design strategies for C-phycocyanin purification: Process influence on purity grade

Amarante, Marina Campos Assumpção de; Braga, Anna Rafaela Cavalcante; Sala, Luisa; Moraes, Caroline Costa; Kalil, Susana Juliano

doi:10.1016/j.seppur.2020.117453

Cited by 35 publications

(9 citation statements)

References 67 publications

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“…The latter value means that the purity of PC increased six-fold with respect to that in the crude extract after dialysis + precipitation, showing the suitability of the IL-based extraction process with this purification approach. In addition, the purity of PC obtained through this purification process was significantly higher than the values previously reported by other authors. ,− For instance, Kumar et al obtained a similar PC purity (2.93) but a lower PC recovery (around 39%). High-purity PC of 3.25 and 3.74, similar to the present work, have been only reported using additional purification stages based on one- or two-step chromatography, respectively .…”

Section: Resultsmentioning

confidence: 51%

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Sánchez-Laso

Espada

Rodríguez

et al. 2023

Ind. Eng. Chem. Res.

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A new biorefinery from Arthrospira platensis was proposed to obtain phycocyanin (PC) and a biocrude by hydrothermal liquefaction (HTL). PC is a high-added-value phycobiliprotein widely used as a food colorant and in the nutraceutical and pharmaceutical industries. However, the use of conventional solvents in the extraction process and the purity grade of the extract are shortcomings in bioproduct production. PC was extracted using a reusable ionic liquid [EMIM][EtSO4], achieving a PC purity of the lowest commercial grade. Therefore, two downstream processes were applied: (1) dialysis + precipitation and (2) aqueous two-phase system (ATPS) + dialysis + precipitation. After the second purification process, the PC purity increased remarkably to reach the analytical grade for pharmaceutical and nutraceutical applications. The waste biomass (WB) obtained in the PC extraction was valorized by hydrothermal liquefaction (HTL) to produce a biocrude. The biocrude yield and composition remarkably enhanced using isopropanol at 350 °C as a cosolvent.

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Section: Resultsmentioning

confidence: 51%

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Sánchez-Laso

Espada

Rodríguez

et al. 2023

Ind. Eng. Chem. Res.

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“…Among the most used and described methods, as shown in Table 4, is a combination of precipitation with ammonium sulphate (AS), membrane filtration (MF), and chromatography (Figure 5). The AS precipitation is a simple and cheap method to improve the recovery and purity of phycocyanin [44,202]. Different concentrations of AS have been evaluated, and the better purity (2.11) and recovery values (86%) of phycocyanin were obtained with 50-65% AS [197,[203][204][205].…”

Section: Phycocyanin Purification Methodsmentioning

confidence: 99%

“…After dialysis and IEC, the purity of phycocyanin increased to 4.05-5.59 and recoveries were 67% [197]. Other authors performed precipitation with 50% AS followed by dialysis, ultrafiltration with a molecular weight cut-off (MWCO) of 50 kDa, and IEC with pH gradient [44] or linear salt gradient elution [202]. They achieved purity values above 4.0, but the phycocyanin yield was higher in the study using IEC with a pH gradient (80% vs. 68%) [41,189].…”

Section: Phycocyanin Purification Methodsmentioning

confidence: 99%

“…Moreover, the phycocyanin purity index will determine its market value and, consequently, its applicability. Indeed, phycocyanin is considered food, cosmetic, reagent, or analytic grade when A 620 /A 280 is, respectively, greater than 0.7, 1.5, 3.9, or 4.0 [44]. The commercial value of phycocyanin can vary from 25 €/mg when partially purified to 200 €/mg for phycocyanin purity of 3.5 [45].…”

Section: Biological Functions and Applicabilitymentioning

confidence: 99%

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Exploring the Benefits of Phycocyanin: From Spirulina Cultivation to Its Widespread Applications

Fernandes¹,

Campos²,

Serra³

et al. 2023

Pharmaceuticals

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Large-scale production of microalgae and their bioactive compounds has steadily increased in response to global demand for natural compounds. Spirulina, in particular, has been used due to its high nutritional value, especially its high protein content. Promising biological functions have been associated with Spirulina extracts, mainly related to its high value added blue pigment, phycocyanin. Phycocyanin is used in several industries such as food, cosmetics, and pharmaceuticals, which increases its market value. Due to the worldwide interest and the need to replace synthetic compounds with natural ones, efforts have been made to optimize large-scale production processes and maintain phycocyanin stability, which is a highly unstable protein. The aim of this review is to update the scientific knowledge on phycocyanin applications and to describe the reported production, extraction, and purification methods, including the main physical and chemical parameters that may affect the purity, recovery, and stability of phycocyanin. By implementing different techniques such as complete cell disruption, extraction at temperatures below 45 °C and a pH of 5.5–6.0, purification through ammonium sulfate, and filtration and chromatography, both the purity and stability of phycocyanin have been significantly improved. Moreover, the use of saccharides, crosslinkers, or natural polymers as preservatives has contributed to the increased market value of phycocyanin.

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“…Phycocyanin has been shown to have antioxidant, anti-obesity, and anti-cancer activities. Phycocyanin is proven to have antioxidants against the ABTS •+ and peroxyl radicals for 161.66 µmolTE.g −1 and 1211.41 µmolTE.g −1 , respectively [7]. Phycocyanin exhibited anti-obesity effects in high-fat diet mice by reducing 39.8±9.7% of body weight, lowering 23.8±1.6% of serum glucose, and decreasing 20.8±1.4% of total cholesterol, while the positive drug Simvastatin had the corresponding values: 8.3±4.6%, 24.8±1.9%, and 1±0.2%, respectively [8].…”

Section: Introductionmentioning

confidence: 99%

Improvement of phycocyanin stability at different temperatures using microencapsulation by whey protein isolate

Tedjakusuma,

Widyaningrum

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Phycocyanin is a blue pigment in cyanobacteria known for its antioxidant activity and can be applied as a natural food colorant. However, one drawback to applying phycocyanin in food products is their high-temperature instability. In this research, we investigated the effect of encapsulation of phycocyanin using whey protein isolate (WPI) as the wall material to improve high-temperature stability. The study also assessed the physiochemical properties of microencapsulated phycocyanin. Phycocyanin was extracted from dry biomass Spirulina using a cold maceration method. Then, phycocyanin extract was encapsulated with prepared emulsions containing 0.25%, 0.50%, 0.75%, and 1.00% WPI as wall materials. The result showed all microencapsulated phycocyanin, regarding various concentrations of WPI, showed lower phycocyanin degradation at 60°C and 70°C at various heating times compared to control, suggesting higher stability. The control sample had 35.55±0.33% and 62.61±0.55% concentration degradation at 60°C and 70°C after 10 min heating. The microencapsulated phycocyanin with 0.50% WPI had 12.67±2.08% and 19.95±2.02% at 60°C and 70°C after 10 min heating. The encapsulation efficiency achieved 98-99% regarding various concentrations of WPI. There was no significant difference in solubility between the control and microencapsulated phycocyanin. Our result concluded that microencapsulation, using WPI as wall material, improved the high-temperature stability of phycocyanin.

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Design strategies for C-phycocyanin purification: Process influence on purity grade

Cited by 35 publications

References 67 publications

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Exploring the Benefits of Phycocyanin: From Spirulina Cultivation to Its Widespread Applications

Improvement of phycocyanin stability at different temperatures using microencapsulation by whey protein isolate

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