This paper aims at investigating the structural response and predicting the ultimate strength of the cold-formed built-up I-section columns affected by local, distortional, global and in particular by the local-distortional (LD) interactive and local-distortional-global (LDG) interactive buckling modes. For this purpose, a total of 18 single C-section columns and 18 built-up I-section columns were tested under uniaxial compression load, respectively. The cross-sectional dimension, the thickness and the length of the tested members were varied in the test so as to cover a wide range of local, distortional and overall slenderness. It was shown in the test that noticeable LD interaction was observed for a built-up column with short length as well as LDG interaction for a built-up column with intermediate length. Due to the clear evidence obtained in the test that LD and LDG interactions cause substantial ultimate strength erosion in cold-formed built-up I-section column, a novel direct strength based method was proposed in this paper to quantify such an erosion effect. The validity of the proposed method was then verified by comparing the results obtained from the proposed method with the test results in this paper as well as several other test results in the literature. The comparison results proved that the proposed method can be used successfully in estimating the ultimate strength of cold-formed built-up I-section column affected by pure buckling mode as well as interactive