Design, synthesis and anticervical cancer activity of new benzofuran–pyrazol-hydrazono- thiazolidin-4-one hybrids as potential EGFR inhibitors and apoptosis inducing agents

Design and green synthesis of novel quinolinone derivatives of potential anti-breast cancer activity against MCF-7 cell line targeting multi-receptor tyrosine kinases

“…Cell cycle analysis and apoptosis investigation were carried out by flow cytometry. 39 , 40 MCF-7 cells were seeded at 8 × 104 and incubated at 37 °C, 5% CO 2 overnight. After treatment with the tested compound, for 24 h, cell pellets were collected and centrifuged (300 g, 5 min).…”

Isatin-Hydrazones with Multiple Receptor Tyrosine Kinases (RTKs) Inhibitory Activity and In-Silico Binding Mechanism

“…The EGFR/VEGFR-2 assay kit comes in a convenient 96-well format, with enough purified recombinant EGFR/VEGFR-2 enzyme, EGFR/VEGFR-2 substrate, ATP and kinase buffer 1 for 100 enzyme reactions. The assay was performed according to the protocol supplied from the EGFR/VEGFR-2 kinase assay kit #40321 (BPS Bioscience, San Diego, CA, USA) and #40325 (BPS Bioscience, San Diego, CA, USA), respectively [58,59]. The EGFR/VEGFR-2 activity at a single dose concentration of 10 µM was performed, where the Kinase-Glo MAX luminescence kinase assay kit (Promega#V6071) was used.…”

Development of 3-methyl/3-(morpholinomethyl)benzofuran derivatives as novel antitumor agents towards non-small cell lung cancer cells

“…Among these pharmacological activities, benzofuran derivatives exhibit significant inhibitory carbonic anhydrase 11,12 , antioxidant 13 , anti-Alzheimer's 14 , anti-inflammatory 15 , antibacterial 15,16 , antitubercular 17 , as well as anticancer activities 18 . Benzofurans were reported to exert their antiproliferative activities through diverse mechanisms of cellular proliferation inhibition including apoptosis induction [19][20][21][22][23] and VEGFR-2 inhibitory action [24][25][26] .…”