Design, Synthesis, and Application of Particle-Based Fluorescence Resonance Energy Transfer Sensors for Carbohydrates and Glycoproteins

Abstract

This paper describes the development of novel particle-based fluorescence resonance energy transfer (FRET) sensors to quantify the concentration and monitor the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins. The sensing approach is based on FRET between fluorescein (donor)-labeled lectin molecules, adsorbed on the surface of micrometric polymeric beads, and polymeric dextran molecules labeled with Texas Red (acceptor). The FRET efficiency of the donor-acceptor pair decreases in the presence of carbohydrates or glycoproteins that compete with the Texas Red-labeled dextran molecules on the lectinic binding sites. The inhibitory effect is concentration and time dependent. The sensing technique enables the discrimination between carbohydrates and glycoproteins based on their binding affinity to the FRET sensing particles as well as quantitative analysis of carbohydrates and glycoproteins in aqueous samples. In the future, the newly developed sensors could enable screening glycoprotein-based drugs for their binding affinity toward selective receptors.

Nanoparticle‐Based Energy Transfer for Rapid and Simple Detection of Protein Glycosylation

“…The photoluminescence (PL) quenching of dex-QDs at 605 nm increased as more conA-AuNPs were added. The apparent binding constant (K a ) of 6.7 10 7 m À1 between conAAuNPs and dex-QDs calculated from this result (see the Supporting Information) is similar to the previously reported value of 1.1 10 7 m À1 , [17] which confirms that the PL quenching of dex-QDs occurs specifically by conA-AuNPs. We also checked whether the PL quenching can be prevented by a protein that inhibits the interaction between conA-AuNPs and dex-QDs.…”

Control of emission peaks of X-ray phosphor using dye-silica nanoparticles

“…4, and the fluorescent cascade from the X-ray-irradiated X-ray phosphor to the concentrated-dye-doped silica nanoparticle would occur in the overlapped wavelength. This property can be expected that the concentrated-fluorescent-molecule-doped silica nanoparticles accept the fluorescent cascade [22] from the X-ray-irradiated phosphor and efficiently emit their fluorescence.…”

Optical Chemical Biosensors for High Throughput Screening of Drugs

“…Rosenzweig et al [118,119] described the development of novel particle-based fluorescence resonance energy transfer (FRET) biosensors. They described the fundamentals of FRET in heterogeneous systems and the application of the new sensors in monitoring the binding affinity of carbohydrates and glycoproteins to lectins, which are carbohydrate binding proteins.…”