2005
DOI: 10.1016/j.jelechem.2005.03.026
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Design variations of a polymer–enzyme composite biosensor for glucose: Enhanced analyte sensitivity without increased oxygen dependence

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Cited by 48 publications
(90 citation statements)
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References 69 publications
(119 reference statements)
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“…On average, the J max value, and therefore enzyme loading, 41 for Pt D /GluOx/PPD was 15 times greater than for Pt C /GluOx/PPD. This is consistent with retention of a dome of enzyme solution around the disk tip as it is removed vertically from the GluOx Figure 2.…”
Section: Resultsmentioning
confidence: 87%
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“…On average, the J max value, and therefore enzyme loading, 41 for Pt D /GluOx/PPD was 15 times greater than for Pt C /GluOx/PPD. This is consistent with retention of a dome of enzyme solution around the disk tip as it is removed vertically from the GluOx Figure 2.…”
Section: Resultsmentioning
confidence: 87%
“…41 After rinsing and a settling period at +700 mV in fresh PBS, amperometric calibrations were carried out to determine the apparent Michaelis-Menten parameters (J max and K M (Glu); see below) and the linear region sensitivity (0-100 µM) of the biosensors to Glu and H 2 O 2 in quiescent air-saturated buffer, unless stated otherwise. All electropolymerizations and calibrations were performed in a standard three-electrode glass electrochemical cell containing 20 mL PBS at room temperature.…”
Section: Methodsmentioning
confidence: 99%
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“…These new determinations for Pt C are in line with other populations determined previously. 41,51 The similarity between the AA slopes for Pt C and Pt D electrodes demonstrates that neither subtle differences in the radial diffusion profiles for these two geometries nor the different condition of the metal surface (pristine cut for Pt D vs the Teflon-stripped origin of the Pt C cylinder; see Figure 1) 44,85 significantly affected AA sensitivity for the bare electrodes under the recording conditions used.…”
Section: Resultsmentioning
confidence: 96%
“…Different values of J max , determined under the same conditions, reflect differences in the amount of active enzyme on the surface, provided the sensitivity of the electrode to H 2 O 2 (reaction (3)) does not vary, as was the case for the PPDmodified Pt cylinders and disks used here. [33][34][35] …”
Section: Introductionmentioning
confidence: 99%