In view of the great importance of the pyrimidine ring and its entry into many aspects of life, including aspects of medical and vital importance, where it was known to be a part of the synthesis of the amino acid histidine, in addition to its entry into the composition of many treatments such as anti-tumor , inflammatory and anti-fungal. Including that the electronic density of this ring is concentrated in the two sites containing the two nitrogen atoms, and then it becomes clear that when a suitable electrophile is available, it can bind with the imidazole ring in one of the two mentioned sites. It has been customary to prove the structures and specificity of the prepared ligands through chemical automated means to diagnose ligands, and then conduct vital studies for them to evaluate their effectiveness and all ligands are multi dentate .The synthesized ligands are type (Imidazole , pyrimidine , sulphone) that have high effect on DNA of bacteria , we noted that Ligands [6 ,4] have high activity towards resistance of bacteria due to their ability to inhibition wall of bacterial cell then kill it for this reasone these ligands have more activity, also these ligands involved sulphone group in its structure.