Designed Fabrication of Polymer-Mediated MOF-Derived Magnetic Hollow Carbon Nanocages for Specific Isolation of Bovine Hemoglobin

Abstract

It is highly required to develop well-designed separation materials for the specific isolation of certain proteins in proteomic research. Herein, the new type of metal–organic framework (MOF)-derived polymer-mediated magnetic hollow nanocages was fabricated via stress-induced orientation contraction, which was further applied for specific enrichment of proteins. The core–shell nanocomposites comprised of polymer-mediated ZIF-67 cores and polydopamine (PDA) shells, after annealing, generated magnetic hollow carbon nanocages with hierarchical pores and structures. Particularly, the magnetic carbonized PDA@F127/ZIF-67 hollow nanocages exhibited a remarkable adsorption capacity toward bovine hemoglobin (BHB) up to 834.3 mg g–1, which was significantly greater than that of the directed carbonized ZIF-67 nanoparticles. The results also exhibited the notable specificity of the obtained nanocages on complex biosamples, including intact mixed proteins and fetal calf serum. The hierarchically hollow porous structure greatly improves the specific surface area and reduces the mass transfer resistance, leading to enhanced high adsorption for target protein BHB. This novel method will be promising for the applications in purification and enrichment of biomacromolecules for complex biosamples, which successfully solve the problem of low adsorption efficiency and tedious separating process of the previous MOF-derived materials.

Cationic Covalent Organic Framework as an Ion Exchange Material for Efficient Adsorptive Separation of Biomolecules

“…Recently, frameworks with metals as part of their architecture have been reported for protein separation. − Despite their capability in protein adsorption, both the lower stability and the possibility of metal leaching have made metal-free organic frameworks preferable for adsorption purposes. In addition, traditional ion exchange media have adsorptive groups bound to the heterogeneous material such as Sepharose, which is a cross-linked polysaccharide displaying different binding properties due to polydispersity and distribution of functional groups on the structure.…”

A One-Stone-Two-Birds Strategy to Functionalized Carbon Nanocages

“…However, the formation of CNCs via the direct carbonization of MOFs remains a great challenge. For instance, the formation of CNCs via the direct carbonization of MOFs involves the use of organic ligands such as polyvinylpyrrolidone (PVP), − polydopamine (PDA), and pyrrole, which may lead to the aggregation of CNCs after carbonization due to the strong binding effects between the functional groups of surface ligands.…”

“…However, the formation of CNCs via the direct carbonization of MOFs remains a great challenge. For instance, the formation of CNCs via the direct carbonization of MOFs involves the use of organic ligands such as polyvinylpyrrolidone (PVP), 11−14 polydopamine (PDA), 15 and pyrrole, 16 which may lead to the aggregation of CNCs after carbonization due to the strong binding effects between the functional groups of surface ligands. In this work, we developed a one-stone-two-birds strategy for the construction of functionalized N-doped CNCs (NCNCs), in which the selective use of hexacarbonyl molybdenum (Mo(CO) 6 ) can not only protect the profile of the zeolitic imidazolate framework-8 (ZIF-8) precursor from collapse during thermal treatment, but also be sacrificed for the functionalization of NCNCs.…”

Surface engineering of personal protective equipments (PPEs) to prevent the contagious infections of SARS-CoV-2

“…Thus a surface with moderate wettability can be obtained by tuning the duration of surface modification. DA coated surfaces are reported to enhance the adsorption capacity of proteins [58,59].…”