Designer Foods: Scope for Enrichment With Microbe-Sourced Antioxidants

Sarethy, Indira P.; Pan, Sharadwata

doi:10.1016/b978-0-12-811520-6.00014-3

Cited by 2 publications

(1 citation statement)

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“…Consequently, research on bioactive peptides continues to grow and, among other important databases, BIOPEP already contains over 3,680 biologically active peptides derived mostly from food sources (Dziuba, Minkiewicz, Nałecz, & Iwaniak, ). The sources (plant, animal, and microbial), production, isolation techniques, and advantages and shortcomings of pursuing food‐derived bioactive peptides as nutraceutical compounds have been discussed in the recent literature (Agyei, Pan, Acquah, & Danquah, ; Aluko, b; Gnasegaran et al, ; Li‐Chan, ; Sarethy & Pan, ; Udenigwe, ; Udenigwe & Aluko, ).…”

Section: Introductionmentioning

confidence: 99%

Structure-informed separation of bioactive peptides

et al. 2019

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The application of proteomic and peptidomic technologies for food-derived bioactive peptides is an emerging field in food sciences. These technologies include the use of separation tools coupled to a high-resolution spectrometric and bioinformatic tools for prediction, identification, sequencing, and characterization of peptides. To a large extent, one-dimensional separation technologies have been extensively used as a continuous tool under different optimized conditions for the identification and analysis of food peptides. However, most one-dimensional separation technologies are fraught with significant bottlenecks such as insufficient sensitivity and specificity limits for complex samples. To address this limitation, separation systems based on orthogonal, multidimensional principles, which allow for the coupling of more than one analytical separation tool with different operational principles, provide a higher separation power than one-dimensional separation tools. This review describes the structure-informed separation and purification of protein hydrolyzates to obtain peptides with desirable bioactivities. Practical applicationsApplication of bioactive peptides in the formulation of functional foods, nutraceuticals, and therapeutic agents have increasingly gained scholarly and industrial attention. The bioactive peptides exist originally in protein sources and are only active after hydrolysis of the parent protein. Currently, several tools can be configured in one-dimensional or multidimensional systems for the separation and purification of protein hydrolyzates. The separations are informed by the structural properties such as the molecular weight, charge, hydrophobicity or hydrophilicity, and the solubility of peptides. This review provides a concise discussion on the commonly used analytical tools, their configurations, advantages and challenges in peptide separation. Emphasis is placed on how the structural properties of peptides assist in the separation and purification processes and the concomitant effect of the separation on peptide bioactivity. K E Y W O R D S bioactive peptides, multidimensional separation, peptide purification, separation systems, structure-property relationships [Correction added on 30 January 2019: this article has been added to the issue after an inadvertent omission.]

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