Designing a cellulolytic enzyme cocktail for the efficient and economical conversion of lignocellulosic biomass to biofuels

Adsul, Mukund; Sandhu, Simranjeet Kaur; Singhania, Reeta Rani; Gupta, Ravi P.; Puri, S.K.; Mathur, Anshu S.

doi:10.1016/j.enzmictec.2019.109442

Cited by 143 publications

(75 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After pretreatment, the remaining carbohydrates (cellulose and a small amount of hemicelluloses) in the pretreated raw materials are converted into fermentable sugars by a cocktail of enzymes (cellulolytic, hemicellulolytic, auxiliary/ AA9) and proteins (e.g., swollenin; Adsul et al, 2020;Payne et al, 2015). In addition to enzyme and proteins, supplement of surfactant (2% Tween-80 or 1% Silwet L-77) into pretreated Miscanthus biomass has massive boosting effect on hexoses yield (Sun et al, 2017(Sun et al, , 2020.…”

Section: Miscanthus Biomassmentioning

confidence: 99%

Miscanthus: A fast‐growing crop for environmental remediation and biofuel production

Wang

Kong

et al. 2020

GCB Bioenergy

View full text Add to dashboard Cite

show abstract

Section: Miscanthus Biomassmentioning

confidence: 99%

Miscanthus: A fast‐growing crop for environmental remediation and biofuel production

Wang

Kong

et al. 2020

GCB Bioenergy

View full text Add to dashboard Cite

show abstract

“…Moreover, cellulolytic mixtures can be optimized, depending on the type of biomass used, the employed pretreatment, and strain optimization [43][44][45]. Studies on the fine-tuning of cellulolytic cocktails are elsewhere reviewed [46].…”

Section: Cellulolytic Cocktailsmentioning

confidence: 99%

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Contreras

Pramanik

Рожкова

et al. 2020

IJMS

View full text Add to dashboard Cite

Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass requires a synergistic action of several cellulases and hemicellulases. Cellulases depolymerize cellulose, the main polymer of the lignocellulosic biomass, to its building blocks. The production of cellulase cocktails has been widely explored, however, there are still some main challenges that enzymes need to overcome in order to develop a sustainable production of bioethanol. The main challenges include low activity, product inhibition, and the need to perform fine-tuning of a cellulase cocktail for each type of biomass. Protein engineering and directed evolution are powerful technologies to improve enzyme properties such as increased activity, decreased product inhibition, increased thermal stability, improved performance in non-conventional media, and pH stability, which will lead to a production of more efficient cocktails. In this review, we focus on recent advances in cellulase cocktail production, its current challenges, protein engineering as an efficient strategy to engineer cellulases, and our view on future prospects in the generation of tailored cellulases for biofuel production.

show abstract

“…Hydrolytic enzymes are major constituents of commercial enzymatic cocktails used in plant biomass conversion of pretreated lignocellulosic materials (Payne et al, 2015;Adsul et al, 2020). The cellulose and hemicellulose hydrolysis promoted by these enzymatic cocktails is the basis of modern biorefineries.…”

Section: Introductionmentioning

confidence: 99%

“…The cellulose and hemicellulose hydrolysis promoted by these enzymatic cocktails is the basis of modern biorefineries. However, improvement of the performance of these enzymatic cocktails is still necessary to advance cost effective production of plant biomass derivatives (Ellila et al, 2017;Adsul et al, 2020). To date, most of these enzymatic cocktails are developed for polysaccharide conversion to monosaccharides and contain diverse cellulolytic and hemicellulolytic enzymes, including glucoside hydrolases (GHs) and auxiliary activity enzymes, such as lytic polysaccharide monooxygenases (LPMOs), that cause oxidative cleavage of polysaccharides (Payne et al, 2015;Adsul et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…However, improvement of the performance of these enzymatic cocktails is still necessary to advance cost effective production of plant biomass derivatives (Ellila et al, 2017;Adsul et al, 2020). To date, most of these enzymatic cocktails are developed for polysaccharide conversion to monosaccharides and contain diverse cellulolytic and hemicellulolytic enzymes, including glucoside hydrolases (GHs) and auxiliary activity enzymes, such as lytic polysaccharide monooxygenases (LPMOs), that cause oxidative cleavage of polysaccharides (Payne et al, 2015;Adsul et al, 2020). Both enzyme groups can contain carbohydrate-binding modules (CBMs) associated with the catalytic domain that enable efficient adsorption of the enzymes on insoluble polysaccharides, improving the enzymatic catalysis efficiency (Payne et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Secretome of Phanerochaete chrysosporium and Trametes versicolor Grown in Microcrystalline Cellulose and Use of the Enzymes for Hydrolysis of Lignocellulosic Materials

Machado

Valadares

Silva

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The ability of white-rot fungi to degrade polysaccharides in lignified plant cell walls makes them a suitable reservoir for CAZyme prospects. However, to date, CAZymes from these species are barely studied, which limits their use in the set of choices for biomass conversion in modern biorefineries. The current work joined secretome studies of two representative white-rot fungi, Phanerochaete chrysosporium and Trametes versicolor, with expression analysis of cellobiohydrolase (CBH) genes, and use of the secretomes to evaluate enzymatic conversion of simple and complex sugarcane-derived substrates. Avicel was used to induce secretion of high levels of CBHs in the extracellular medium. A total of 56 and 58 proteins were identified in cultures of P. chrysosporium and T. versicolor, respectively, with 78-86% of these proteins corresponding to plant cell wall degrading enzymes (cellulolytic, hemicellulolytic, pectinolytic, esterase, and auxiliary activity). CBHI predominated among the plant cell wall degrading enzymes, corresponding to 47 and 34% of the detected proteins in P. chrysosporium and T. versicolor, respectively, which confirms that Avicel is an efficient CBH inducer in whiterot fungi. The induction by Avicel of genes encoding CBHs (cel) was supported by high expression levels of cel7D and cel7C in P. chrysosporium and T. versicolor, respectively. Both white-rot fungi secretomes enabled hydrolysis experiments at 10 FPU/g substrate, despite the varied proportions of CBHs and other enzymes present in each case. When low recalcitrance sugarcane pith was used as a substrate, P. chrysosporium and T. versicolor secretomes performed similarly to Cellic R CTec2. However, the white-rot fungi secretomes were less efficient than Cellic R CTec2 during hydrolysis of more recalcitrant substrates, such as acid or alkaline sulfite-pretreated sugarcane bagasse, likely because Cellic R CTec2 contains an excess of CBHs compared with the white-rot fungi secretomes. General comparison of the white-rot fungi secretomes

show abstract

Designing a cellulolytic enzyme cocktail for the efficient and economical conversion of lignocellulosic biomass to biofuels

Cited by 143 publications

References 96 publications

Miscanthus: A fast‐growing crop for environmental remediation and biofuel production

Miscanthus: A fast‐growing crop for environmental remediation and biofuel production

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

The Secretome of Phanerochaete chrysosporium and Trametes versicolor Grown in Microcrystalline Cellulose and Use of the Enzymes for Hydrolysis of Lignocellulosic Materials

Contact Info

Product

Resources

About