Designing a fully automated multi‐bioreactor plant for fast DoE optimization of pharmaceutical protein production

Abstract

Supporting information see http://dx

Optimized Reaction Conditions Enable the Hydration of Non‐natural Substrates by the Oleate Hydratase from Elizabethkingia meningoseptica

“…Reaction conditions were further improved by using MODDE as a tool for statistical experiment design . MODDE enables the simultaneous analysis of several factors to identify critical parameters, synergistic effects, and suitable operating conditions by statistical evaluation.…”

Model-Based Design of Process Strategies for Cell Culture Bioprocesses: State of the Art and New Perspectives

“…Even though the prediction of the resin's porosity was of insufficient accuracy, the simulation allowed deeper understanding of the process, which leads to a more efficient determination of the Design Space. In the general procedure of process development, the use of complex, demanding, and time-consuming methods based on DoE tools has become common practice, although mathematical models are already used in the operation, control, and optimization of existing production processes [47,58,59]. Novel tools for process development and optimization combine DoE tools with a growth model.…”

Sequential/parallel production of potential Malaria vaccines – A direct way from single batch to quasi-continuous integrated production

“…Fig. 2 shows how this is divided into a 10 h lasting exponential growth phase where P. pastoris grows on glycerol and dilutes and re-consumes the whole cell internal content of target protein P 1 and AOX which is remaining from the previous breeding cycle (Fricke et al, 2013a). In the following 14 h lasting pre-induction phase these cells where re-prepared for a protein expression in the production bioreactor by feeding and controlling methanol as a new carbon source.…”