Designing a Peptide‐modified Screen‐printed Gold Electrode as a Sensor for the Human Monocytic Leukemia Cell Line

Abstract

We designed a screen‐printed gold electrode (SPGE) with a peptide to detect cancer cells. The ability to sense circulating tumor cells (CTCs) and monitor their movement in blood vessels is important for cancer diagnosis. Human monocytic leukemia cells were selected as a model of CTCs because of their origin. The cell‐recognition and cell‐sensing peptide moieties were EICADP and Y4C. The SPGE was modified with the cell‐recognition moieties of the CY4EICADPY4C in a bridge‐type construction due to the cysteine residue at both terminals. The calibration curve was linear and ranged from 25 to 2,000 cells/mL with a LOD of 8 cells/mL.

Electrochemical sensing of target cells based on a peptide/single‐strand DNA probe

“…Ferrocene and methylene blue are widely used as labels, and a change in the electrode response of the label is based on the interaction of the peptide with a target [24]. We also performed electrochemical sensing for cells [25] and protein [26] using molecular recognition/electron-transfer peptides. In this case, peptides that consist of tyrosine and cysteine residues are powerful as electron-transfer elements.…”