Designing and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human samples

Raoufi, Ehsan; Hosseini, Fatemeh; Onagh, Bahman; Salehi-Shadkami, Mohammad; Mehrali, Marjan; Mohsenzadegan, Monireh; Ho, Jim Q.; Bigdelou, Banafsheh; Sepand, Mohammad Reza; Webster, Thomas J.; Zanganeh, Saeid; Farajollahi, Mohammad M

doi:10.1016/j.cca.2023.117279

Cited by 2 publications

(1 citation statement)

References 38 publications

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“…Coating assay plates with recombinant protein is the first step in any conventional ELISA technique in which adsorption occurs via hydrophobic interactions of the diluted antigen to the surface of the well [ 32 , 33 ]. This approach is mostly used to detect pathogen-specific antibodies in a clinical sample and provides high accuracy [ 34–41 ].…”

Section: Discussionmentioning

confidence: 99%

tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain

Bernauer,

Maier,

Bannert

et al. 2024

Biology Methods and Protocols

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Enzyme-linked immunosorbent assay (ELISA) systems use plates coated with peptides or expressed and purified proteins to monitor immunoglobulins derived from patient serum. However, there is currently no easy, flexible, and fast adaptive ELISA-based system for testing antibodies directed against new SARS-CoV-2 variants. In this study, we utilized the tANCHOR protein display system that provides a cell surface decorated with the receptor binding domain (RBD) to monitor specific antibodies derived from SARS-CoV-2 convalescent and vaccinated individuals directed against it. To test sera from vaccinees or convalescent individuals, only the RBD coding sequence needs to be cloned in the tANCHOR vector system and transfected into HeLa cells. Time-consuming protein expression, isolation, and purification followed by coating assay plates is not necessary. With this technique, the immune evasion of new SARS-CoV-2 variants from current vaccination regimes can be examined quickly and reliably.

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Section: Discussionmentioning

confidence: 99%

tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain

Bernauer,

Maier,

Bannert

et al. 2024

Biology Methods and Protocols

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Sex-specific outcomes in cancer therapy: the central role of hormones

Bakhshi,

Ho,

Zanganeh

2024

Front. Med. Technol.

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Sex hormones play a pivotal role in modulating various physiological processes, with emerging evidence underscoring their influence on cancer progression and treatment outcomes. This review delves into the intricate relationship between sex hormones and cancer, elucidating the underlying biological mechanisms and their clinical implications. We explore the multifaceted roles of estrogen, androgens, and progesterone, highlighting their respective influence on specific cancers such as breast, ovarian, endometrial, and prostate. Special attention is given to estrogen receptor-positive (ER+) and estrogen receptor-negative (ER−) tumors, androgen receptor signaling, and the dual role of progesterone in both promoting and inhibiting cancer progression. Clinical observations reveal varied treatment responses contingent upon hormonal levels, with certain therapies like tamoxifen, aromatase inhibitors, and anti-androgens demonstrating notable success. However, disparities in treatment outcomes between males and females in hormone-sensitive cancers necessitate further exploration. Therapeutically, the utilization of hormone replacement therapy (HRT) during cancer treatments presents both potential risks and benefits. The promise of personalized therapies, tailored to an individual’s hormonal profile, offers a novel approach to optimizing therapeutic outcomes. Concurrently, the burgeoning exploration of new drugs and interventions targeting hormonal pathways heralds a future of more effective and precise treatments for hormone-sensitive cancers. This review underscores the pressing need for a deeper understanding of sex hormones in cancer therapy and the ensuing implications for future therapeutic innovations.

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Designing and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human samples

Cited by 2 publications

References 38 publications

tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain

tANCHOR cell-based ELISA approach as a surrogate for antigen-coated plates to monitor specific IgG directed to the SARS-CoV-2 receptor-binding domain

Sex-specific outcomes in cancer therapy: the central role of hormones

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