Upper respiratory tract infections are the major causes of mortality and morbidity for children under 5 years. The aims of this study to develop and apply an educational program for nursery school supervisors based on their needs to improve their knowledge and practice about prevention of upper respiratory tract infections among children under 5 years old. A quasi-experimental Research Design was used to conduct this study which carried out at (12) nursery schools from east and west of Assiut city. The subjects included a convenient sample of (57) participants (35) teachers and (22) supervisors Two tools were used, structured questionnaire and observation checklist which were developed by the researcher. Results of this study revealed that, there was statistically significant difference between total mean score of studied participants knowledge and practice related to upper respiratory tract infection between pretest and posttests (1)and(2) p. value (0.001 ,0.009) (0.000, 0.000) respectively. And there were statistically significant differences between studied Participants knowledge and their Socio demographic characteristics. The study Concluded that the studied participant knowledge, practices regarding the prevention of upper respiratory tract infections for children under five years in nursing schools in Assiut city were improved after the implementation of the educational program. The study Recommended providing periodic regular training educational program for all nursery schools teachers, and supervisors about infection control precautions.