Designing Inherently Photodegradable Cell‐Adhesive Hydrogels for 3D Cell Culture

Rosenfeld, Alisa; Göckler, Tobias; Kuzina, Mariia; Reischl, Markus; Schepers, Ute; Levkin, Pavel A.

doi:10.1002/adhm.202100632

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…For example, we have previously demonstrated that PEGMA/PEGDMA hydrogels that do not expose any bioactive sites yielded cell viability around 10% only. However, when supplemented with 1.25% GelMA, viability remarkably increased to over 60% [51] . Furthermore, we did not observe agglomeration or spheroid formation of HepG2 cells as it usually occurs in PEO hydrogels [52] .…”

Section: Cytocompatibility Of Gelma/bpe Bioinksmentioning

confidence: 95%

Polyelectrolyte Complex Hydrogel Scaffoldings Enable Extrusion-based 3D Bioprinting of Low-Viscosity Bioinks

Göckler

Grimm

et al. 2023

Preprint

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We generate self-assembled biocompatible scaffolds with excellent structural integrity based on complex-forming block polyelectrolytes that enable extrusion-based 3D bioprinting of large constructs from low-viscosity bioinks. Despite remarkable progress of biofabrication techniques in tissue engineering, the development of extrudable bioinks that perform optimally at physiological temperatures remains a major challenge. Most biopolymer and photocurable precursor solutions exhibit low viscosities at 37 °C, resulting in undesirable flows and loss of form prior to chemical crosslinking. Temperature-sensitive bioinks, such as gelatin methacryloyl (GelMA), can be deposited near their gelling point, but suffer from suboptimal temperature-induced pre-gelation, poor cell viability emerging from long holding times in the cooled cartridges, inefficient temperature transfer from the print bed, and discontinuous layer-by-layer fabrication. Here, we demonstrate that block polyelectrolyte additives serve as effective viscosity enhancers when added to non-extrudable precursor solutions. Rapid, electrostatic self-assembly of block polyelectrolytes into either jammed micelles or interconnected networks provides hydrogel scaffoldings that form nearly instantly, lend initial structural robustness upon deposition, and enhance shear and tensile strength of the cured bioinks. Moreover, our approach enables continuous extrusion without the need of chemical crosslinking between individual layers, paving the way for fast biomanufacturing of human-scale tissue constructs with improved inter-layer bonding.

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Section: Cytocompatibility Of Gelma/bpe Bioinksmentioning

confidence: 95%

Polyelectrolyte Complex Hydrogel Scaffoldings Enable Extrusion-based 3D Bioprinting of Low-Viscosity Bioinks

Göckler

Grimm

et al. 2023

Preprint

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“…They demonstrate that UV irradiation of such polymethacrylates can cause photohomolysis of main-chain carbon–carbon bonds resulting in the degradation of the hydrogel . On the basis of this technology, they developed an inherently photodegradable poly(ethylene glycol) methacrylate (PEGMA)-based material, supplemented with GelMA to increase biocompatibility and make it suitable for 3D cell culture . Similarly, Applegate and colleagues developed another photopatterning technique completely without the use of any exogenous or chemical cross-linkers.…”

Section: Hydrogel Patterning By Means Of Photodegradation Photorelaxa...mentioning

confidence: 99%

“…115 On the basis of this technology, they developed an inherently photodegradable poly(ethylene glycol) methacrylate (PEGMA)-based material, supplemented with GelMA to increase biocompatibility and make it suitable for 3D cell culture. 116 Similarly, Applegate and colleagues developed another photopatterning technique completely without the use of any exogenous or chemical cross-linkers. They created 2D and 3D multiscale patterns in soft silk hydrogels by means of ultrafast laser pulses.…”

Section: Hydrogel Patterning By Means Of Photodegradation Photorelaxa...mentioning

confidence: 99%

Stimuli-Responsive Hydrogels: The Dynamic Smart Biomaterials of Tomorrow

Neumann,

di Marco,

Iudin

et al. 2023

Macromolecules

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“…The possibility of adjusting hydrogel stiffness by external stimuli such as pH, temperature, crosslinking, magnetic or light exposure allows the replication of diverging tumor tissues. 44 It is thereby possible to modulate migration, proliferation and drug sensitivity of the embedded cells. The synthetic hydrogels are superior to biological hydrogels with respect to reproducibility, scalability and tunability.…”

Section: Tissue Architecturementioning

confidence: 99%