Interval Training is the newly used training program because of its effectiveness in developing both the aerobic and lactate energy systems. However, there are controversies due to different kinds of training program in choosing and using them to trains an athlete. Therefore,this study was aimed to see theeffect of 6-week new interval training protocol on running performance of MIE track trainee athletes. Depending on scientific evidence researchers hypothesized that there will be significant difference between pre and post test performance for middle (800m, 1500m) and long(3Km,5Km &10Km) distance runner. Longitudinal experimental research design was used; pertaining to data collection pre and post and athletes performed interval training 6-8sessions over 6 weeks conducted on 15 male and 15 female elite athletes. Statistical analysis such as percentages, mean, standard deviation paired sample t-test, tables and figures by entering the data into SPSS version-20.Thirty elite athletes of age: 18±2yrs; height 167 ± 5.8centimeter; weight 51.2± 4.29Kg. Significance difference was observed at P<0.05. The athletes showed significant performance improvement in their respective events.