Designing stable, hierarchical peptide fibers from block co-polypeptide sequences

Rijt, Mark M. J. van; Ciaffoni, Adriano; Ianiro, Alessandro; Moradi, Mohammad-Amin; Boyle, Aimee L.; Kros, Alexander; Friedrich, Heiner; Sommerdijk, Nico A. J. M.; Patterson, Joseph P.

doi:10.1039/c9sc00800d

Cited by 10 publications

(9 citation statements)

References 60 publications

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“…Nanotube formation was also observed in a series of amphiphilic block co-polypeptides Ac-[ALV] x - b -[KGE] y -NH 2 ( x + y = 7) (Van Rijt et al ., 2019). TEM analysis indicated that peptide Ac-[ALV] 3 - b -[KGE] 4 -NH 2 self-assembled to form high aspect-ratio nanotubes of ~90 Å in diameter that were stable over a wide pH range (pH = 2–12) and at temperatures up to 80 °C.…”

Section: Cross-β Nanotubesmentioning

confidence: 99%

Structures of synthetic helical filaments and tubes based on peptide and peptido-mimetic polymers

Miller

Hughes

Modlin

et al. 2022

Quart. Rev. Biophys.

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Synthetic peptide and peptido-mimetic filaments and tubes represent a diverse class of nanomaterials with a broad range of potential applications, such as drug delivery, vaccine development, synthetic catalyst design, encapsulation, and energy transduction. The structures of these filaments comprise supramolecular polymers based on helical arrangements of subunits that can be derived from self-assembly of monomers based on diverse structural motifs. In recent years, structural analyses of these materials at near-atomic resolution (NAR) have yielded critical insights into the relationship between sequence, local conformation, and higher-order structure and morphology. This structural information offers the opportunity for development of new tools to facilitate the predictable and reproducible de novo design of synthetic helical filaments. However, these studies have also revealed several significant impediments to the latter processmost notably, the common occurrence of structural polymorphism due to the lability of helical symmetry in structural space. This article summarizes the current state of knowledge on the structures of designed peptide and peptido-mimetic filamentous assemblies, with a focus on structures that have been solved to NAR for which reliable atomic models are available. Table of contents Cross-β nanotubes 12 Coiled-coil filaments 19 Tandem repeat assemblies 26 Foldamer-based helical assemblies 29 Conclusions 33 2 Jessalyn G. Miller et al. 4 Jessalyn G. Miller et al.

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Section: Cross-β Nanotubesmentioning

confidence: 99%

Structures of synthetic helical filaments and tubes based on peptide and peptido-mimetic polymers

Miller

Hughes

Modlin

et al. 2022

Quart. Rev. Biophys.

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“…Thermodynamically controlled processes tend to result in predictable and well-defined structures. However, their application is limited by their sensitivity to changes in the environment, which can significantly alter the formed structures 7,8 . Kinetically controlled processes tend to result in stable structures, but the final structures can be highly sensitive to the assembly method 5,7,9 .…”

mentioning

confidence: 99%

“…However, their application is limited by their sensitivity to changes in the environment, which can significantly alter the formed structures 7,8 . Kinetically controlled processes tend to result in stable structures, but the final structures can be highly sensitive to the assembly method 5,7,9 . While the final structural dependence on assembly methodology offers the flexibility to form multiple hierarchical structures from a single BCP, it necessitates strict control of the synthetic conditions 3,7,10 .…”

mentioning

confidence: 99%

Ring-opening polymerization-induced crystallization-driven self-assembly of poly-L-lactide-block-polyethylene glycol block copolymers (ROPI-CDSA)

2020

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The self-assembly of block copolymers into 1D, 2D and 3D nano- and microstructures is of great interest for a wide range of applications. A key challenge in this field is obtaining independent control over molecular structure and hierarchical structure in all dimensions using scalable one-pot chemistry. Here we report on the ring opening polymerization-induced crystallization-driven self-assembly (ROPI-CDSA) of poly-L-lactide-block-polyethylene glycol block copolymers into 1D, 2D and 3D nanostructures. A key feature of ROPI-CDSA is that the polymerization time is much shorter than the self-assembly relaxation time, resulting in a non-equilibrium self-assembly process. The self-assembly mechanism is analyzed by cryo-transmission electron microscopy, wide-angle x-ray scattering, Fourier transform infrared spectroscopy, and turbidity studies. The analysis revealed that the self-assembly mechanism is dependent on both the polymer molecular structure and concentration. Knowledge of the self-assembly mechanism enabled the kinetic trapping of multiple hierarchical structures from a single block copolymer.

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“…Peptide secondary structure has been shown to influence the self-assembly of peptide-containing conjugates; [27,[32][33][34][35] this is further confounded by the ability of steric crowding to induce secondary structure in peptides. [28,36] Therefore, to focus on the effect of peptide hydrophilicity on self-assembly we used two classes of unstructured peptides-PAS [37] and XTEN [38][39][40] -and from these, selected a total of four sequences that vary in length and charge density (Figure 1b, Figures S11-S14).…”

Section: Scope Of Design Space and Synthesis Of Ppasmentioning

confidence: 99%

Mapping the Morphological Landscape of Oligomeric Di‐block Peptide–Polymer Amphiphiles**

et al. 2022

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Peptide-polymer amphiphiles (PPAs) are tunable hybrid materials that achieve complex assembly landscapes by combining the sequence-dependent properties of peptides with the structural diversity of polymers. Despite their promise as biomimetic materials, determining how polymer and peptide properties simultaneously affect PPA self-assembly remains challenging. We herein present a systematic study of PPA structure-assembly relationships. PPAs containing oligo(ethyl acrylate) and random-coil peptides were used to determine the role of oligomer molecular weight, dispersity, peptide length, and charge density on self-assembly. We observed that PPAs predominantly formed spheres rather than anisotropic particles. Oligomer molecular weight and peptide hydrophilicity dictated morphology, while dispersity and peptide charge affected particle size. These key benchmarks will facilitate the rational design of PPAs that expand the scope of biomimetic functionality within assembled soft materials.

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Designing stable, hierarchical peptide fibers from block co-polypeptide sequences

Abstract: Here we report the pH induced self-assembly of equilibrium zwitterionically charged block co-polypeptide nanotubes into hierarchical nanotube fibers.

Cited by 10 publications

References 60 publications

Structures of synthetic helical filaments and tubes based on peptide and peptido-mimetic polymers

Structures of synthetic helical filaments and tubes based on peptide and peptido-mimetic polymers

Ring-opening polymerization-induced crystallization-driven self-assembly of poly-L-lactide-block-polyethylene glycol block copolymers (ROPI-CDSA)

Mapping the Morphological Landscape of Oligomeric Di‐block Peptide–Polymer Amphiphiles**

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