Designing Triangular Silver Nanoplates with GSH/GSSG Surface Mixed States as Novel Nanoparticle-based Redox Mediators for Electrochemical Biosensing

Abstract

Herein, a dual signal-quenched electrochemical (EC) biosensing strategy utilizing surface-engineered trisodium citrate (TSC)− glutathione (GSH)/oxidized glutathione (GSSG)-capped triangular silver nanoplates (Tri-Ag NPs TSC−GSH/GSSG ) as a novel nanoparticle-based redox mediator was explored for biomarker determination. In contrast with conventional redox mediators, Tri-Ag NPs TSC−GSH/GSSG provided more admirable EC performance along with a lower oxidation potential (∼0.14 V). Taking advantage of the split-type mode, the immune response in a 96well microplate was independent from EC detection, which could effectively eliminate the biological interference and thereby greatly enhance the sensitivity. As for the surface engineering process of Tri-Ag NPs, it was composed of partial GSH replacement and the formation of the GSH/ GSSG surface mixed state. Primarily, the signal response of Ag NPs TSC−GSH decreased due to the hindrance of GSH on electron transfer. Moreover, varying proportions of GSH/GSSG could further impede the oxidation process of Tri-Ag NPs TSC−GSH/GSSG and eventually realize efficient dual signal quenching of this system. Notably

Heterojunction MnO2-nanosheet-decorated Ag nanowires with enhanced oxidase-like activity for the sensitive dual-mode detection of glutathione

“…[1][2][3] Upon oxidation, GSH can be converted into the GSSG form. 4,5 As an antioxidant, GSH participates in a variety of cellular processes including intracellular signal transduction, maintenance of protein structure, gene transcription regulation, and immune response. 6,7 However, an abnormal level of GSH or the GSH/GSSG ratio is closely related with some diseases, such as tumors, and neurological, metabolism, and blood diseases.…”

Nanoassemblies Based on a Cationic Perylene Diimide Derivative and Sodium Dodecyl Sulfate: A Simple Fluorescent Platform for Efficient Analysis of Aflatoxin B1

“…Aflatoxins (AFs) are a group of secondary metabolites of low molecular weight produced by Aspergillus flavus and Aspergillus parasiticus, which could contaminate grains and other agricultural commodities. , Due to the capacity of binding to cellular DNA, aflatoxin B 1 (AFB 1 ) is one of the most carcinogenic compounds found in nature and possesses the highest toxicity among aflatoxins, resulting in significant health risk and damage to humans and animals. − So far, many countries have established the maximum residue levels (MRLs) of AFB 1 in the range of 2–20 μg kg –1 . , The high stability of AFB 1 to physical and chemical treatments enables their survival through these treatments including various food manufacturing processes at elevated temperatures, making it exceedingly challenging to prevent their entry into the food chain. , Therefore, accurate and sensitive determination of AFB 1 residues in agricultural commodities is highly needed.…”

Engineering Entropy-Driven Nanomachine-Mediated Morphological Evolution of Anisotropic Silver Triangular Nanoplates for Colorimetric and Photothermal Biosensing

“…For example, there have been many photothermal biosensing reported based on aggregated Au nanoparticles, Au nanostars, and Au nanorods . Compared to their wide application in colorimetric/electrochemical sensors or electrochemical catalysis, − anisotropic Ag nanostructures demand more exploration in photothermal sensing. Since the vertex is highly active and rich in Ag + ions, anisotropic Ag triangular nanoplates (Ag TNPs) tend to change from triangular to sphere via etching process, accompanying with the large plasmon peak shift .…”